Background: Diabetic nephropathy (DN) is one of the most well-known diabetic microvascular complications, affecting around 40% individuals with type 2 diabetes mellitus (T2DM). It progresses to end-stage renal disease (ESRD), and its primary detection can be done via biomarkers. The power early biomarker identification DN in T2DM serum evaluated this study. Aim: This study aimed assess possibil...

Bu çalışma 2019-2021 yılları arasında Antalya ili marul üretim alanlarında iri damar virüsü (Mirafiori lettuce big vein virus: MiLBVV)’nün belirlenmesi amacı ile yürütülmüştür. Arazi çalışmalarında 240 örneği toplanmış ve bütün örnekler MiLBVV’ye spesifik antiserumlar kullanılarak DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunosorbent Assay) yöntemi testlenmiştir. Testlenen bitki örnek...

A leading application of biomolecular interaction analysis technology is the characterization of antibodyantigen interactions. By measuring the association and dissociation rates of the interaction, antibodies with desired binding characteristics can be identified. A rapid association rate and a slow dissociation rate may be indicative of therapeutic efficacy. Affinity maturation programs exist...

Monoclonal antibody against P. mameffei yeast secreted antigen was produced in order to develop a serological test for penicilliosis marneffei. The yeast form of P. marneffei was cultured in brain heart infusion broth at 37 degrees C for 7 days. A secreted antigen was prepared, partially purified from culture supernatant and subsequently immunized in a BALB/c mouse. Mouse monoclonal antibody wa...

The ability of Bacillus cereus to cause foodborne toxicoinfections leads to increasing concerns regarding consumer protection. For the diarrhea-associated enterotoxins, the assessment of the non-hemolytic enterotoxin B (NheB) titer determined by a sandwich enzyme immunoassay (EIA) correlates best with in vitro cytotoxicity. In general, the regulation of enterotoxin expression of B. cereus is a ...

We have previously reported that human anti-mouse IgG antibody (HAMA) can cause false-positive and false-negative results in "sandwich"-type monoclonal antibody (MAb) assays. To eliminate HAMA interference in "sandwich"-type MAb assays, we investigated the use of MAb on solid-phase, vinylidene fluoride floccules, which we have previously used as a solid-phase second antibody for RIA. The simple...