A serine proteinase (Alp) from the culture supernate of a clinical isolate of Aspergillus fumigatus was purified to virtual homogeneity at a yield of 41%. The procedure involved affinity chromatography on agarose-epsilon-amino-caproyl-D-tryptophan methyl ester. Alp had an estimated mol. wt of 32 Kda and the pI was determined at pH 7.9. The enzyme was fully inhibited by phenylmethyl sulphonyl fl...

Article history: Received on: 16/05/2016 Revised on: 10/06/2016 Accepted on: 20/06/2016 Available online: 05/11/2016 In plants, pollen tube germination occurs widely in flowering plants. In Arabidopsis thaliana, it has been reported ARABINOGALACTAN PROTEIN 1 (AGP1) plays an important role in pollen tube germination. The expression of arabinogalactan protein in Arabidopsis pollen tubes has been ...

A useful laboratory test for the differentiation of liver, bone, and intestinal alkaline phosphatase (ALP) isoenzymes in serum is presented. Electrophoresis in polyacrylamide gel is performed with untreated serum as well as with serum incubated at 56 degrees C for 10 min. The heating step denatures bone isoenzyme which may obscure the liver ALP band when present in large amounts. Visualization ...

Antileucoprotease (ALP), a potent inhibitor of human neutrophil elastase (HNE), may be a modulating factor in the pathogenesis of emphysema. Investigating the clearance of intratracheally-instilled ALP in hamsters, we observed a rapid clearance from the airway lumen within 60 min, whereafter the remaining 40% slowly decreased with a calculated half-life (T0.5) of 2.8 h. Lung tissue-associated A...

the activity of mouse endometrial alkaline phosphatase after hyperstimulation and progesterone injection at the implantation time alkaline phosphatase (alp) of endometrium may play a critical function in the development and implantation of embryo. the aim of this study was to determine the localization of endometrial alp activity after hyperstimulation and progesterone injection. thirty adult f...

conclusions in issns, bone strength recovered one month after terminating stx. in igan, bone strength recovered 10 months after tapering of stx. results in issns, the bmd and s-alp levels were significantly lower in phase 1 than in phase 0; however, scores and levels returned to baseline in phase 2. in igan, bmd was significantly lower in phase 2 than in phase 0 while s-alp levels were signific...

The ubiquitin-proteasome system (UPS) and autophagy-lysosome pathway (ALP) are the two most important mechanisms that normally repair or remove abnormal proteins. Alterations in the function of these systems to degrade misfolded and aggregated proteins are being increasingly recognized as playing a pivotal role in the pathogenesis of many neurodegenerative disorders such as Parkinson's disease....

Aluminum phosphide (AlP) is used throughout the world as pesticides to protect stored grains from rodents and other pests (Cienki, 2001). The chemical is usually formulated in pellets, granules or as a dust. Upon contact with moisture in the environment, AlP undergoes a chemical reaction yielding phosphine gas (PH3), which is the active pesticidal component and a very toxic system poison, makes...

On the basis of the inhibition of double strand DNA (dsDNA)-templated fluorescent copper nanoparticles (CuNPs) by pyrophosphate (PPi), a novel label-free turn-on fluorescent strategy to detect alkaline phosphatase (ALP) under physiological conditions has been developed. This method relies on the strong interaction between PPi and Cu(2+), which would hamper the effective formation of fluorescent...

Abnormalities of bone mineral metabolism and vascular calcification are prevalent in patients with kidney failure. Clinical management is based on biochemical targets, in particular parathyroid hormone (PTH) concentrations, but this has many limitations including high biological variation. A possible alternative is bone-specific alkaline phosphatase (ALP); therefore, we evaluated the biological...