We have developed a new helper-dependent (HD) adenoviral vector FTC that contains 3 cis-acting sequences as stuffer DNA: a human fragment of alphoid repeat DNA, matrix-attachment regions (MARs), and the hepatocyte control region enhancer. To determine the most robust human coagulation factor IX (hFIX) expression cassette in an adenovirus, we first tested different hFIX expression sequences with...

Bcl-xs is a dominant negative repressor of Bcl-2 and Bcl-xL, both of which inhibit apoptosis. We used a replication-deficient adenoviral vector to transiently overexpress Bcl-xs in MCF-7 human breast cancer cells, which overexpress Bcl-xL. Infection with this vector induced apoptosis in vitro. We then determined the effects of intratumoral injection of bcl-xs adenovirus on solid MCF-7 tumors in...

"Cell type-and region-dependent coxsackie adenovirus receptor expression in the central nervous system." Abstract Model systems have shown that adenoviral vector mediated transient gene expression can potentially be applied for the treatment of brain tumours, neurodegenerative diseases and brain injuries. Most studies utilized adenovirus serotype 5 (Ad5) based vectors, which as adhesion molecul...

Conventional parenteral injection of vaccines is limited in its ability to induce locally-produced immune responses in the respiratory tract, and has logistical disadvantages in widespread vaccine administration. Recent studies suggest that intranasal delivery or vaccination in the respiratory tract with recombinant viral vectors can enhance immunogenicity and protection against respiratory dis...

PREVIOUS studies using adenoviral vectors in experimental liver transplant settings have demonstrated in vivo infectivity by perfusing grafts with viral vectors. 1•2 However. these techniques required high viral titers (1 to 5 X 1010 plaque-forming units [pfu)) to induce effective viral infection. In this study, we developed an improved gene delivery method that allows near maximal infectivity ...

Background Levels of circulating tumor cells (CTCs) have been associated with overall survival (OS) in metastatic breast [1-] and prostate cancer []. Here we report the preliminary associations between CTCs and OS in subjects receiving an autologous adenoviral transduced dendritic cell (DC) vaccine expressing human HER2 extracellular (EC) and transmembrane (TM) domains (AdHER2ECTM) in adults wi...