Interplasmidic and intrachromosomal recombination in Deinococcus radiodurans has been studied recently and has been found to occur at high frequency following exposure to ionizing radiation. In the current work, we document plasmid-chromosome recombination following exposure of D. radiodurans to 1.75 Mrad (17.5 kGy) 60Co, when the plasmid is present in the cell at the time of irradiation. Recom...

Changes in bamboo composition and microstructure following 60Co gamma (γ) ray irradiation were investigated by solid state 13C crosspolarization (CP) magic-angle spinning (MAS) spectroscopic nuclear magnetic resonance spectrometry (NMR) and a field emission scanning electron microscope (FESEM). The results indicated that irradiation doses lower than 100 KGy resulted in the degradation of hemice...

Synthetic A-type carbonated apatite prepared in controlled conditions was irradiated at room temperature with 60Co gamma rays. The ESR spectrum was associated to axial CO2- and orthorhombic CO3- species. Radicals used as dose markers in biological apatites are long-lived paramagnetic species. The stability of the post-irradiation signal of A-type apatite was investigated for almost 2 years. Mea...

Calf thymus histone octamer complexes were irradiated in the native state in N2O-saturated dilute aqueous solution (0.5 g/l, pH 9, [NaClO4] = 1 -4 mol/l) with 50 or 100 ns pulses of 16 MeV electrons or 60Co-gamma-rays. Time resolved light scattering measurements and optical absorption measurements yielded the following: the octamers underwent a volume contraction due to intra-complex-crosslinki...

Lethal effects of thermal neutrons were compared with those of 60Co-ƒÁ-rays on the two types of Amoeba proteus. D37 doses against ƒÁ-rays were 2.5 •~ 105 (M-type) and 7.2 •~ 105 rads (P type), and those for thermal neutrons were 9.1 and 11.7 •~ 1013 n. cm-2 (total flux) respectively. Additions of AET, D2O or boron failed to increase or decrease the lethal effects of thermal neutrons. The lethal...

Genetic engineering of radiation-resistant organisms to recover radionuclides/heavy metals from radioactive wastes is an attractive proposition. We have constructed a Deinococcus radiodurans strain harboring phoN, a gene encoding a nonspecific acid phosphatase, obtained from a local isolate of Salmonella enterica serovar Typhi. The recombinant strain expressed an approximately 27-kDa active Pho...

Prototype Si microstrip sensors for the CDF-II ISL were fabricated by Hamamatsu Photonics and SEIKO Instruments using 4A technology. The sensor is AC coupled and double-sided forming a stereo angle of 1.2073. The strip pitch is 112 lm on both sides. The main di!erences between the two manufacturers lie on the technologies of passivation and the structure of coupling capacitors. We describe the ...