cDNA clones encoding human factor V have been isolated from an oligo(dT)-primed human fetal liver cDNA library prepared with vector Charon 21A. The cDNA sequence of factor V from three overlapping clones includes a 6672-base-pair (bp) coding region, a 90-bp 5' untranslated region, and a 163-bp 3' untranslated region within which is a poly(A) tail. The deduced amino acid sequence consists of 222...

background: expansion of gaa trinucleotide repeats is the molecular basis of friedreich’s ataxia (frda). precise detection of the gaa expansion repeat in frataxin gene has always been a challenge. different molecular methods have been suggested for detection of gaa expansion, including; short-pcr, long-pcr, triplet repeat primed-pcr (tp-pcr) and southern blotting. the aim of study was to evalua...

This study aims to find out the influence of planting distance on growth and yield peanut crops optimal distances that can increase growth. research was conducted in Keppo Village Pamekasan District Galis November 2021 - January 2022. The design uses a RandomIzed Group Design (RAK) which consists 5 types systems with 4 repeats so there are 20 plots treatment. Data has been obtained using divers...

Trinucleotide repeats (TNRs) undergo frequent mutations in families affected by TNR diseases and in model organisms. Much of the instability is conferred in cis by the sequence and length of the triplet tract. Trans-acting factors also modulate TNR instability risk, on the basis of such evidence as parent-of-origin effects. To help identify trans-acting modifiers, a screen was performed to find...

INTRODUCTION Syncope and palpitations as the only initial manifestations of myotonic dystrophy type 1 (MD1) due to a CTG expansion of 50-100 repeats have not been reported. CASE REPORT In a 55-year-old female with a family history of MD1 and a personal history of a single syncope, palpitations, and hyperCKemia, 70 CTG repeats were detected in the DMPK gene. Her brother had presented atypical ...

This study analyzed the karyotype of Cyrtodactylus inthanon Kunya et al., 2015 from Doi Inthanon, Chiang Mai Province, northern Thailand. The metaphase and meiotic chromosome preparations were obtained by squash technique bone marrow testes, respectively. chromosomes stained Giemsa staining, Ag-NOR banding molecular cytogenetics with ?uorescence in situ hybridization (FISH) using microsatellite...

Filamins are actin binding proteins that contribute to cytoskeletal integrity and biochemical scaffolds during mechanochemical signal transductions. Structurally, human filamins are dimers composed of an actin-binding domain with 24 immunoglobulin (Ig)-like repeats. In this study, we focus on the recently solved high-resolution crystal structure of Ig-like repeats 19-21 of filamin-A (IgFLNa-R19...

There is a high incidence of esophageal squamous cell carcinoma (ESCC) in Iran. Non-functionality of some tumor suppressor genes has been reported in esophageal cancer. Loss of heterozygosity on chromosome 5 has also been reported in esophageal carcinomas. We assessed loss of heterozygosity along a region of the long arm of chromosome 5 (5q), from 5q23.1 to 5q23.2, by PCR amplifying DNA fr...