AIM Chronic organ-donor shortage has led to the acceptance of steatotic livers for transplantation, despite the higher risk of graft dysfunction or nonfunction associated with the ischemic preservation period of these organs. The present study evaluates the effects of trimetazidine (TMZ) on an isolated perfused liver model. METHODS Steatotic and non-steatotic livers were preserved for 24 h in...

Microarray transcriptome analyses of fetal mouse liver did not detect circadian expression rhythms of clock genes or clock-controlled genes, although some rhythmic transcripts that were likely not driven by endogenous cellular clocks were identified. This finding reveals a key distinction between the circadian oscillators in fetal and adult mouse livers. Thus, in this study, the transcriptomes ...

Background: This study was performed to evaluate the levels of heavy metals in the livers and kidneys of cattle, sheep and goats from a mining region in the west of Iran. Methods: In this cross-sectional study, 90 samples were collected from the livers and kidneys of cattle (n=30), sheep (n=30) and goats (n=30) from a slaughterhouse in Hamedan City, western Iran. Lead, cadmium, manganese, zi...

AIMS Ethanol constricts hepatic vessels of isolated perfused livers of rats, but not dogs. However, it is not known whether ethanol constricts or dilates the hepatic vessels in other species such as guinea pigs and mice. In addition, the sites of hepatic venoconstriction induced by ethanol were not known in rat livers. We therefore studied the effects of ethanol on the segmental hepatic vascula...

The fetal liver ¡sozymes aldolase A and pyruvate kinase K increase in livers of adult rats fed a choline deficient-diet contain ing 0.1% ethionine. Oval cells isolated by centrifugal elutriation from preneoplastic livers of animals receiving the carcinogenic diet contained these fetal forms as well as fetal-adult isozyme hybrids. In contrast, parenchymal cells isolated from the livers of these...

BACKGROUND/AIM To determine the effect of marginal donor livers on mortality and graft survival in liver transplantation (LT) recipients. MATERIALS AND METHODS Donors with any 1 of following were considered marginal donors: age ≥65 years, sodium level ≥ 165 mmol/L and cold ischemia time ≥ 12 h. Donors were classified according to the donor risk index (DRI) < 1.7 and ≥ 1.7. The transplant reci...

1. Sulphur amino acid metabolism in livers from rats which had been fed ad lib. on a restricted diet (malnourished) or a Zn-deficient diet was investigated. Perfusion of normal livers with different amounts of L-methionine revealed that homocysteine was recycled four to five times before being eliminated via the transsulphuration pathway. 2. From the perfusion experiments, we found no evidence ...

Fatty liver is a significant risk factor for liver transplantation, and accounts for nearly half of the livers rejected from the donor pool. We hypothesized that metabolic preconditioning via ex vivo perfusion of the liver graft can reduce fat content and increase post-transplant survival to an acceptable range. We describe a perfusate medium containing agents that promote the defatting of hepa...

Normothermic machine perfusion has previously been demonstrated to restore damaged warm ischemic livers to transplantable condition in animal models. However, the mechanisms of recovery are unclear, preventing rational optimization of perfusion systems and slowing clinical translation of machine perfusion. In this study, organ recovery time and major perfusate shortcomings were evaluated using ...