The threonine dehydrogenase (TDG) pathway is a significant route of threonine degradation, yielding glycine in experimental animals, but has not been accurately quantitated in humans. Therefore, the effect of a large excess of dietary threonine, given either as free amino acid (+Thr) or as a constituent of protein (+P-Thr), on threonine catabolism to CO(2) and to glycine was studied in six heal...

The potentiation of glycine-induced responses by ethanol (EtOH) was studied in neurons freshly dissociated from the ventral tegmental area (VTA) of 5- to 14-day-old postnatal rats using whole-cell and gramicidin-perforated patch-clamp techniques. Under current-clamp conditions, EtOH increased glycine-induced membrane depolarization and action potential firing. Under voltage-clamp conditions, Et...

butunoate. glutamate, leucine, isoleucine, phenylalanine. threonine and valine; the organic acids glyoxylate, glycollate, oxalate, succinate, malonate, malate, citrate and glycerate; the fatty acids palmitate and stearate; the sterols campesterol, stigmasterol and sitosterol; the nitrogenous compounds urea, cytosine and uridine; and the poly-hydroxy compounds glycerol, a-glycerophosphatc. /I-gl...

The clinical associations of glycine receptor antibodies have not yet been described fully. We identified prospectively 52 antibody-positive patients and collated their clinical features, investigations and immunotherapy responses. Serum glycine receptor antibody endpoint titres ranged from 1:20 to 1:60 000. In 11 paired samples, serum levels were higher than (n = 10) or equal to (n = 1) cerebr...

To determine whether GABA and glycine can act as cotransmitters at synapses in the rat spinal cord, we have compared the ultrastructural distribution of GABAA-receptor beta 3 subunit with that of the glycine receptor-associated protein gephyrin and combined this with postembedding detection of GABA and glycine. We also used a dual-immunofluorescence method to confirm that gephyrin was associate...

Neural activity in intact chorda tympani nerve of rats was studied with an electronic summator. Neural activity increased when amino acid solutions 0.01 M or above passed over the tongue. Response magnitude, at concentrations close to solubility limits for the amino acids tested, was: DL-methionine < DL-tryptophan < DL-valine < DL-alanine < glycine < 0.1 M NaCl. Maximum response magnitudes to 1...

We present a method to determine concentrations o f glycine between 1 and 3 mM in the simultaneous presence o f 2 5 -lO O m M glutamate, which is a modification o f the procedure originally described by Klein and Linser, Z. Physiol. Chem. 205, 251 (1932). Glycine and glutamate react with 150 m M o-phthaldialdehyde, glycine is quantitatively con­ verted into a colored o-phthaldialdehyde-glycine-...

objective: the aim of this study was to estimate genetic variability and phonetic relationships of 50 isolates of p. sojae from iran. methods: during 2005–2007, 142 isolates of phytophthora sojae were collected from soil samples and diseased soybean plants from lorestan, mazandaran, golestan and ardabil provinces. races of the isolates were determined. dna of p. sojae fifty isolates was amplifi...

the aluminum oxide materials are widely used in ceramics, refractories and abrasives due to their hardness, chemical inertness, high melting point, non-volatility and resistance to oxidation and corrosion. the paper describes work done on synthesis of α-alumina by using the simple, non-expensive solution combustion method using glycine as fuel.aluminum oxide (al2o3) nanoparticles were synthesiz...

Several compounds that promote activation of the N-methyl-d-aspartate receptor (NMDAR) glycine site have been proposed as treatments for schizophrenia, but the impact of these putative antipsychotics on anxiety remains unclear. In this study, we employed genetic and pharmacological mouse models of altered NMDAR glycine site function to examine the effects of these proposed treatments in uncondi...