In recent years, environmental pollution by coal mining is a long-established human activity affecting all levels of life with various environmental impacts by generating heavy metals. The presence of heavy metals even in trace amount is toxic and detrimental to all living organisms. The coal mine area in Bokaro is one of the “Toxic Hotspot” in India. Bacteria have evolved uptake and efflux mec...

Many hospital antimicrobial stewardship programs restrict the availability of selected drugs by requiring prior approval. Carbapenems may be among the restricted drugs, but it is unclear if hospitals that restrict availability actually use fewer carbapenems than hospitals that do not restrict use. Nor is it clear if restriction is related to resistance. We evaluated the relationship between car...

The synthesis and antibacterial activity of the title compounds having an isoxazolidine ring at the C-2 position are described. These derivatives were synthesized by the 1,3-dipolar cycloaddition reaction of nitrone with 2-vinyl carbapenems. This 1,3-dipolar cycloaddition reaction proceeded regioselectively to give diastereomeric isomers of 2(-isoxazolidin-5-yl)carbapenems. It was ascertained t...

Acinetobacter baumannii is an aerobic non-motile Gram-negative coccobacillus, and it is one of the most important nosocomial pathogens worldwide. The aim of this study was to determine the molecular epidemiology of the outbreak strains. Between March 2011 and March 2014, a total of 205 strains of A. baumannii were isolated from patients at the Nanyang City Center Hospital. The blaOXA-23, blaOXA...

Knowledge regarding the genomic structure of Enterobacter spp., the second most prevalent carbapenemase-producing Enterobacteriaceae, remains limited. Here we sequenced 97 clinical Enterobacter species isolates that were both carbapenem susceptible and resistant from various geographic regions to decipher the molecular origins of carbapenem resistance and to understand the changing phylogeny of...

Introduction: The adequate protocol for treatment of an infection is often determined on the basis minimum inhibitory concentration (MIC) causative organism. Traditional methods (agar dilution, microbroth and gradient diffusion) are labour intensive time consuming (they usually take over 48 hours to report results). On other hand, automated systems (VITEK™, Phoenix™, MicroScan WalkAway™) rapid ...

134 P&T® • March 2008 • Vol. 33 No. 3 INTRODUCTION Carbapenems represent a class of beta-lactam antibacterial agents with a broad spectrum of activity against grampositive, gram-negative, and anaerobic bacteria. This class is bactericidal in nature, but it does not cover species of Enterococcus faecium, methicillin-resistant Staphylococcus aureus (MRSA), or Stenotrophomonas maltophilia. Collect...

Patients of all ages and genders regularly experience urinary tract infections. Antibiotic misuse has caused organisms to develop drug resistance, creating a treatment problem. Objective: To uncover risk variables relationships, as well the antibiotic susceptibility in UTI patients. Methods: Diabetes non-diabetes with were compared within groups using Chi-square test, significant P-value 0.05. ...

The genus Enterobacter comprises a range of beneficial plant-associated bacteria showing plant growth promotion. Enterobacter ludwigii belongs to the Enterobacter cloacae complex and has been reported to include human pathogens but also plant-associated strains with plant beneficial capacities. To assess the role of Enterobacter endophytes in hydrocarbon degradation, plant colonization, abundan...

This study provides evidence of direct transfer of functional DNA from species to species, specifically Escherichia coli pGFPuv and Enterobacter aerogenes. An Escherichia coli made pGFPuv invasive by transformation via miniprep transfers DNA after simple co-incubation with Enterobacter aerogenes. Transfer efficiency was enhanced or disturbed in the bacteria by altering pH. Expression of the acq...