The single-electrode voltage-clamp technique requires sharp glass-capillary microelectrodes, whose electrical properties often limit the capabilities of the recording system. Here, we describe a rapid and simple way of coating fine microelectrodes with Dricote and Vaseline that improves their performance during voltage-clamp. The coating prevented clogging of the tips, improved the capacitance ...

Two new electrostatic discharge (ESD) protection design by using only 1 × VDD low-voltage devices for mixedvoltage I/O buffer with 3 × VDD input tolerance are proposed. Two different special high-voltage-tolerant ESD detection circuits are designed with substrate-triggered technique to improve ESD protection efficiency of ESD clamp device. These two ESD detection circuits with different design ...

1. Inactivation of delayed outward current was studied by voltage clamp of isolated neurone somata of the molluscs Archidoris and Anisodoris. During prolonged voltage clamp steps in normal artificial sea water delayed outward current rises to a peak and then declines to a non-zero steady-state. During repetitive clamp pulses at repetition rates slower than 2/sec, the amplitude of peak outward c...

Electrophysiological techniques make use of Ag/AgCl electrodes that are in direct contact with cells or bath. In the bath, electrodes are exposed to numerous experimental conditions and chemical reagents that can modify electrode voltage. We examined voltage offsets created in Ag/AgCl electrodes by exposure to redox reagents used in electrophysiological studies. Voltage offsets were measured in...

1. We describe a new method, the dynamic clamp, that uses a computer as an interactive tool to introduce simulated voltage and ligand mediated conductances into real neurons. 2. We simulate a gamma-aminobutyric acid (GABA) response of a cultured stomatogastric ganglion neuron to illustrate that the dynamic clamp effectively introduces a conductance into the target neuron. 3. To demonstrate an a...

Abstract The X-ray inspection of wire clamps is used more and widely. detection uses UAV (Unmanned Aerial Vehicle) to inspect the clamp with high voltage, which directly contacts electrified clamp. When strength overhead transmission line exceeds air breakdown strength, extreme discharges will occur, so control data performance influenced, even cause a crash. electromagnetic interference can al...

Voltage-clamp fluorometry (VCF) supplies information about the gating transitions of voltage-gated proteins. Changes in fluorescence intensity dye attached to a part protein that undergoes conformational rearrangement upon alteration membrane potential by electrodes constitute signal. The VCF signal is generated quenching and dequenching as traverses various local environments. Here we studied ...

TASAKT, I. AND C. S. SPYROPOULOS. Nonunijorm response in the squid axon membrane under ‘voltage-clamp.’ Am. J. Physiol. 193(2): 309-317. 1958.-By cooling a portion of the squid giant axon at a time, it was shown that the repetitive membrane currents observed under the so-called voltageclamp conditions derived from the main portion of the ‘clamped’ axon membrane and not from the lateral unclampe...