A neurophysiological detector (NPD) is a hybridization of olfactory system neurons of the fish crucian carp, Carassius carassius L., with a computerized electronic device connected to a high-performance liquid chromatograph (HPLC). This system makes it possible to measure neurophysiological activities in the olfactory system of C. carassius L. after exposure of this fish to alarm pheromones. Th...

Hemicellulose is a component of Non-Starch Polysaccarides (NSP) contained in agricultural byproduct and can be separated from the other components. Various studies about hemicelluloses isolation procedures from various agricultural byproduct materials have been conducted including corn cobs. Objective of this research is to modify the isolation method and compare with other procedures of isolat...

Cocoa bean is a resource with great level of bioactive components that have shown potential beneficial effects on health, in addition to being the main ingredient chocolate industry. This study evaluated total antioxidant capacity (quencher-DPPH ° ), polyphenols, fatty acid profile, and chromatic parameters Peruvian commercial cocoa beans. The different analytes were quantified using UV–Vis abs...

Roots of Withania somnifera are widely used in Indian herbal formulations. Withaferine A and withanolide A are two pharmacologically important withanolides present in the roots of the plant. HPLC method using DAD and ELSD detector was developed for simultaneous determination of withaferine A and withanolide A from the roots and herbal formulation of the plant. Coarse powder of root was extracte...

The aim of this study was to evaluate the production of dried fruits and juices from chokeberry as potential sources of bioactive compounds with beneficial effects on human health. Dry powders and juices from chokeberry were analyzed for the contents of sugars with high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD method), and the antioxidan...

The most important developments in propolis analysis and pharmacological properties are discussed. In order to help in the Romanian propolis standardization, different methodologies for chemical composition analysis (UV-VIS, HP-TLC, and HPLC-DAD) are reviewed using new approaches and software (fuzzy divisive hierarchical clustering approach and ChromQuest software) and compared with internation...

Electrochemical detection of sugar-related compounds was conducted using a boron-doped diamond (BDD) electrode as a detector for flow-injection analysis (FIA). Sugar-related compounds oxidize at high applied potentials, for which the BDD electrode is suitable for electrochemical measurements. Conditions for an FIA system with a BDD detector were optimized, and the following detection limits wer...

Background: Nicotine is the most used toxic substance in spite of mass media awareness. Column estimation nicotine found to be inhibiting factor for its estimation.Objective: To develop a method on C8 column HPLC.Methods: The involved model Waters 1515 binary HPLC Pump system interfaced with 2489 UV/VIS detector, 4.6 X 250 mm, 5μm beads Symmetry at 37°C, and an isocratic mobile phase containing...

A method for the determination of phosphorylation sites in phosphoproteins based on column-switching high-performance liquid chromatography (HPLC) has been developed. The HPLC system consisted of a titania precolumn for the selective adsorption of phosphopeptides, an anion-exchange analytical column and a UV detector (215 nm). Rabbit muscle phosphorylase a (RPa) and porcine stomach pepsin (PSP)...