Objective: Peel of Amorphophallus is considered as waste. In this study we checked for the presence of cellulase enzyme in this waste and investigated its biochemical properties. Methods: Presence of cellulase activity was confirmed by Congo red plate diffusion assay The optimum temperature, pH, substrate concentration for both CMCase and FPase activity was also determined by DNS method. Effect...

In today's world, there is an increasing trend towards the use of renewable, cheap and readily available biomass in the production of a wide variety of fine and bulk chemicals in different biorefineries. Biorefineries utilize the activities of microbial cells and their enzymes to convert biomass into target products. Many of these processes require enzymes which are operationally stable at high...

In-ovo vaccination is an attractive immunization approach for poultry industry. However, most of the Newcastle disease virus (NDV) vaccine strains used after hatch are unsafe, as in-ovo vaccines, due to their high pathogenicity for chicken embryos. In this study, we evaluated the safety and immunogenicity of a thermostable NDV strain TS09-C, derived from V4 strain, as in-ovo vaccine. Chickens i...

Enhancing cocaine metabolism by administration of cocaine esterase (CocE) has been recognized as a promising treatment strategy for cocaine overdose and addiction, because CocE is the most efficient native enzyme for metabolizing the naturally occurring cocaine yet identified. A major obstacle to the clinical application of CocE is the thermoinstability of native CocE with a half-life of only a...

There is a high demand for engineering thermostable enzymes in some industries; especially in paper industries to use environmental friendly enzymes instead of toxic chlorine chemicals. Hence, understanding protein attributes involved in enzyme thermostability is important. Herein, the most important protein features contributing to enzyme thermostability was searched by using data mining algor...

Establishing a good algorithm for predicting temperature of thermostable proteins is an important issue. In this study, a new thermostable proteins prediction method by using Hurst exponent and Choquet integral regression model with respect to maximized L-measure is proposed. The main idea of this method is to integrate the physicochemical properties, long term memory property and Choquet integ...