Cytokines are a group of peptides which form a sophisticated network to modulate multiple cellular events. Within such a network, and through complex feedback mechanisms, cytokine functions are largely interdependent and closely associated with a number of pathological processes. In the present study, the EVIDENCE 180 system was used to study the ...

Saccharopolyspora erythraea acyl-carrier protein, highly expressed from a T7-based expression plasmid in Escherichia coli, can be selectively released from the cells in near-quantitative yield by a single cycle of freezing and thawing in a neutral buffer. Electrospray mass spectrometry was used to confirm that the recombinant S. erythraea acyl-carrier protein over-expressed in E. coli is presen...

Shallow slides in cohesionless till occurred in several highway fills in the province of Quebec, Canada, following spring thaw and sometimes during rainy autumns. This study has shown that the strength parameters of cohesionless tills can be related to the liquid limit for both drained and undrained conditions. These relationships can be used as input in stability analyses to readily yield desi...

NADH dehydrogenase of Escherichia coli, a component of the electron transport chain, oxidizes NADH to NAD. Previously, it was demonstrated that when E. coli K12 cells were lysed by the lysozyme lysis method, NADH dehydrogenase activity was inhibited; however, when cells were lysed by French press, NADH dehydrogenase activity was observed. In this study, components commonly used in lysozyme lysi...

Soil compaction is recognized as a yield limiting factor in crop production. Compaction problems continue to exist for several reasons, including; 1) the large size (mass) of agricultural vehicles; 2) increased operations on wet soils because of larger farm acreage and multi-axle driven equipment; and, 3) the lack of controlled traffic patterns in fields. Where compaction is identified as a pro...

The relationship between adenosine deaminase (ADA) and a human membrane thymus leukemia (HTL) antigen-detected by an antithymocyte serum was explored. A freeze-thaw extract of the T-cell-derived cell line, MOLT 4, was applied to an immunoabsorbant column of a rabbit antiserum to calf ADA. The bound MOLT 4 ADA was eluted with 6 M urea. The recovered ADA had a specific activity of 490 mumol of ad...

the cold climate is a favorable parameter for the development of tension cracks and decrease of rock brittleness. therefore, this paper attempts to investigate the hamekasi porous limestone in order to predict the brittleness indices during freeze-thaw cycles. the freeze–thaw test was executed for one cycle including 16 h of freezing, and 8 h of thawing. the geo mechanical properties and brittl...

Service life assessment of three historical building envelopes constructed using different types of sandstone is presented. At first, experimental measurements of material parameters of sandstones are performed to provide the necessary input data for a subsequent computational analysis. In the second step, the moisture and temperature fields across the studied envelopes are calculated for a rep...

Results: The experiment results indicate that the type of recycled aggregate and its replacement ratio have significant effects on the freezethaw performance. The cubic compressive strength of recycled aggregate concrete is overall slightly lower than that of normal concrete. After 100 freeze-thaw cycles, the compressive strength decreases and the reduction extent increases with increasing repl...