The purpose of this report is to compare measurements of enzymatic activities and cell surface markers as methods of distinguishing subtypes of lymphoid leukemias of childhood. Twenty-six children ages 2-15 yr were studied. Terminal deoxynucleotidyl transferase (TdT) activity was high in blasts from all 20 children with either null or T cell acute lymphoblastic leukemia. The activity of adenosi...

Abstract Rationale and Aim of the Study Cardiac involvement is very common in transfusion dependent beta-thalassemia (TDT) mainly related to cardiac iron overload myocardial damage with progressive systolic diastolic dysfunction left right ventricles. It has also been observed that patients TDT, fibrosis may not be directly deposition associated a higher risk cardiovascular complications. CMR g...

Inspired by the chemical synthesis of molecularly imprinted polymers, we demonstrated for first time, protein-target mediated enzyme-generated aptamers (EGAs). We prepared pre-polymerisation mixtures containing different ratios nucleotides, an initiator sequence and protein template incubated each mixture with terminal deoxynucleotidyl transferase (TdT). Upon purification rebinding EGAs against...

Surgical extent-of-resection has been shown to have an impact on high-grade glioma (HGG) outcomes; however, complete resection is rarely achievable in difficult-to-access (DTA) tumors. Controlled thermal damage to the tumor may have the same impact in DTA-HGGs. We report our multicenter results of laser interstitial thermal therapy (LITT) in DTA-HGGs. We retrospectively reviewed 34 consecutive ...

Intracellular adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) and terminal deoxynucleotidyl transferase (TdT) activities were investigated in adult patients with acute myelogenous leukemia (AML) in order to relate these enzymatic activities to the stage of differentiation and maturation and to the clinical outcome of AML. Both ADA and PNP were measured spectrophotometrically us...

The TDT-3 Text and Speech Corpus expands on previous phases of Topic Detection and Tracking data collections, by increasing the number of news sources being sampled, by including Mandarin Chinese as well as English news data, and by introducing new forms of topic annotation. In order to satisfy the specific data and annotation requirements of the TDT-3 Evaluation Plan[1], the LDC refined and su...

We prove an existence of a topological decision tree which solves the range searching problem for a system of real polynomials, in other words, the tree finds all feasible signs vectors of these polynomials, with the (topological) complexity logarithmic in the number of signs vectors. This answers the problem posed in [FK98]. 1 Range Searching Problem Let polynomials f1, . . . , fm ∈ R[X1, . . ...

The mouse thymus contains two forms of terminal deoxynucleotidyl transferase (TdT) which are distinguishable by the salt concentration necessary to elute them from a phosphocellulose column, by their distrubtion among the thymocyte subpopulations, and by their sensitivity to cortisone treatment. In the whole thymus the later eluting peak (peak II) is the predominant one with about 3-10% of the ...

In this paper a microplate method is described for diagnosing acute leukemia and for investigating the reactivity of monoclonal antibodies (MoAbs) against membrane antigens in combination with rabbit or murine antibodies to nuclear terminal transferase (TdT). The speed of this method facilitates the investigation of fresh leukemic cells from individual patients and assesses the cytolytic effica...

T-cell receptor (TCR) beta-chain proteins appear early (approximately 15th week of gestation) during human thymic ontogenesis. These beta-chain proteins, which appear before terminal deoxynucleotidyl transferase (TdT), could be an expression of a fully rearranged (V-D-J), incompletely rearranged (D-J), or germline TCR beta-chain gene. The aims of this study, performed from the 15th week onward,...