Sewage-water treatment comprehends primary, secondary, and tertiary steps to produce reusable water after removing sewage contaminants. However, a sewage-water plant is typically power energy consumer produces high volumes of sludge mainly generated in the primary secondary steps. The use more efficient anaerobic digestion with can reasonable flowrates biogas, which shown be consolidated strate...

By using flow linear dichroism, in combination with nuclease digestion and two spectroscopically distinguishable DNAs, we demonstrate the existence of two internal and one external DNA-binding sites in the RecA fiber. A number of different complexes between RecA and single- and double-stranded DNAs are characterized with respect to stoichiometry, location, and base orientation of each of the as...

A high-throughput sample preparation protocol based on the use of 96-well molecular weight cutoff (MWCO) filter plates was developed for shotgun proteomics of cell lysates. All sample preparation steps, including cell lysis, buffer exchange, protein denaturation, reduction, alkylation and proteolytic digestion are performed in a 96-well plate format, making the platform extremely well suited fo...

Methods nCor a 8 was purified from raw hazelnuts by precipitation and chromatographical steps. Subsequently, the protein was identified by N-terminal sequencing and mass spectrometry. Stability of the protein was investigated by simulated gastric and duodenal digestion assays. Hazelnut lipids were extracted employing the hexane method. Finally, IgE binding activity of native and reduced/alkylat...

a method to quick detect concentration of lecithin in eggs, namely microwave digestion spectrophotometry, was established in this research. the homogenate of eggs was treated with absolute ethanol to eliminate phosphate protein in eggs which could possibly affect concentration of lecithin examined. a sample then received a new way of pre-treatment, called microwave digestion, before uv-vis spec...

The present experiment was carried out to determine the digestion kinetics of carbohydrate fractions of citrus by-products. Grapefruit pulp (GP), lemon pulp (LE), lime pulp (LI) and orange pulp (OP) were the test feed. Digestion kinetic of whole citrus by-products and neutral detergent fiber (NDF) fraction and acid detergent fiber (ADF) fractions of citrus by-products were measured using the <e...

Fluorescence in situ hybridization (FISH) has proven to be a very useful technique for the study of chimerism and microchimerism (2,4). Based on the detection of sex chromosomes, this technique allows for the identification and localization of donor and recipient cells in cases of sex-mismatched transplantation or cells from mothers and their male fetuses in studies of fetomaternal cell traffic...

the present experiment was carried out to determine the digestion kinetics of carbohydrate fractions of citrus by-products. grapefruit pulp (gp), lemon pulp (le), lime pulp (li) and orange pulp (op) were the test feed. digestion kinetic of whole citrus by-products and neutral detergent fiber (ndf) fraction and acid detergent fiber (adf) fractions of citrus by-products were measured using the in...