A highly sensitive nested reverse transcriptase-PCR assay, with primers derived from the prostate-specific antigen (PSA) and prostate specific membrane antigen (PSM) cDNA sequences, has been used to detect occult hematogenous micrometastatic prostate cells. In 77 pa tients with prostate cancer, PSM and PSA primers detected circulating prostate cells in 48 (62.3%) and 7 (9.1 %) patients, respect...

AIMS To evaluate three oligonucleotide primer pairs--two specific for 16S and 23S rRNA sequences of Burkholderia cepacia, and the third specific for internal transcribed spacer region of 16S-23S sequences of B gladioli--for the identification and differentiation of reference and clinical strains of these and other species. METHODS The three primers sets were applied in polymerase chain reacti...

various molecular techniques are available to make differences and to study the genetic divergences among species and populations, of which the most efficient ones are pcr-based markers. one of these methods, molecular fingerprinting, enables the distinction of closely related populations. issr-primers are universal primers that can be used for such studies. the utility of these primers was tes...

A dinuclear nickel complex with methyl and thiolate ligands, Ni(dadt(Et))Ni(Me)(SDmp) (2), has been synthesized as a dinuclear Ni(d)-Ni(p)-site model of acetyl-CoA synthase (ACS) (dadt(Et) is N,N'-diethyl-3,7-diazanonane-1,9-dithiolate; Dmp is 2,6-dimesitylphenyl). Complex 2 was prepared via 2 methods: (i) ligand substitution of a dinuclear Ni(II)-Ni(II) cation complex [Ni(dadt(Et)) Ni(tmtu)2] ...

This service integrates primer designing code from the popular Primer3 1 software with a specificity check that uses a custom BLAST search. Primer-BLAST eliminates the need to design primers at another site and then perform a search with those primers using the main NCBI BLAST service to check specificity. Moreover, Primer-BLAST can design primers that amplify only a particular splice variant o...

BACKGROUND Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein's structure and function. OBJECTIVES We introduced a nested-SOE-PCR (N -SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR. MATERIALS AND METHODS Genomic DNA from Bacillus thermoc...

We established a specific genotyping assay for HLA-A*01, which is one of the most frequently found HLA-A alleles in the Caucasian population. This assay uses the polymerase chain reaction (PCR) with allele group-specific primers (ASP). HLA-A*01 group-specific primers were designed for exon 3 of the HLA-A gene, based on the recent HLA-sequence alignment. Both sense and anti-sense primers were de...

the aim of this study was to detect and differentiate theileria annulata and t. lestoquardi (hirci) by pcr. members of the genus theileria are tick-borne hemoprotozoan parasites those cause fatal and enervating diseases of cattle and sheep in iran . in order to develop a specific method for detecting and identification of theileria species, specific primers from the surface protein (sp) sequenc...