نتایج جستجو برای: soluble e
تعداد نتایج: 1099551 فیلتر نتایج به سال:
Ehrlichia chaffeensis, the etiologic agent of human monocytic ehrlichiosis, replicates in early endosomes by avoiding lysosomal fusion in monocytes and macrophages. In E. chaffeensis we predicted three pairs of putative two-component regulatory systems (TCSs) designated PleC-PleD, NtrY-NtrX, and CckA-CtrA based on amino acid sequence homology. In the present study to determine biochemical pairs...
vitamin e is a potent reactive oxygen metabolites (rom) scavenger. it is a lipid-soluble vitamin and its main function is to protect polyunsaturated fatty acids against oxidative stress. twenty-five mechanically ventilated intensive care unit (icu) adult patients participated in a prospective randomized clinical trial receiving either placebo (10 patients) or 3 im doses (1000 iu each) of vitami...
Selection of a system for successful recombinant protein production is important. The aim of this study wasto produce high levels of human interleukin-2 (hIL-2) in soluble form. To this end, the pET32a vector inEscherichia coli BL21 (DE3) was used as an expression system, since it was previously used for the productionof mouse IL-2 in soluble form. The results indicated that c...
BACKGROUND Bleomycin (BLM), a well known anti-cancer drug, often causes acute lung injury and fibrosis by mechanisms that are not well understood. It is suspected that some proteases and active oxygen species generated from inflammatory cells cause the lung injury and subsequent lung fibrosis. It was therefore hypothesised that inhibition of adhesion of inflammatory cells to the endothelium mig...
It was hypothesized that there would be an upregulation of systemic neutrophil CD11b expression in pneumonia. Expression of CD11b and concentrations of soluble intercellular adhesion molecule (ICAM)-1 and E-selectin were evaluated as potential surrogate markers of the severity of pneumonia. Possible age-related immunosenescence in relation to neutrophil CD11b expression in elderly patients with...
Plasmids containing the foot-and-mouth disease virus structural protein precursor (P1) and 3C protease genes or the P1 gene alone were expressed in Escherichia coli. A recombinant baculovirus containing the P1 gene was also generated and expressed in Spodoptera frugiperda cells. Expression of the P1 and 3C genes in E. coli resulted in efficient synthesis and processing of the structural protein...
2.1. Glutathione 2.1.A. Biological Functions 2.1.B. Biosynthesis 2.1.C. Degradation 2.1.D. Other Thiol-Based Redox Buffers 2.2. Ascorbate 2.2.A. Ascorbate Chemistry 2.2.B. Ascorbate Biosynthesis 2.2.C. Ascorbate Recycling 2.2.D. Ascorbate Transport 2.2.E. Importance of Ascorbate in Stress and Disease 2.3. Other Antioxidants 2.3.A. Lipid-Soluble Antioxidants 2.3.B. Water-Soluble Antioxidants 2.3...
Previously we have shown that high metastatic colonic carcinoma cells express relatively more lamp molecules and sialyl Le(x) structures on the cell surface than their corresponding low metastatic counterparts (Saitoh, O., Wang, W.-L., Lotan, R., and Fukuda, M. (1992) J. Biol. Chem. 267, 5700-5711). In the present study, we extended these findings by testing whether these high and low metastati...
Human vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis and plays a central role in the process of tumor growth and metastatic dissemination. Escherichia coli is one of the most common expression systems used for the production of recombinant proteins; however, expression of human VEGF in E. coli has proven difficult because the E. coli-expressed VEGF tends to be misf...
research was carried out in 2007 on 7 native summer-ripening apple cultivars grown in kamal abad karaj research station. the 16 year-old plants were grafted onto seed rootstocks. the cultivars were ghandak-e-kashan, golab-e-isfahan, golab-e-kohanz, mashad, assali, ghermez-e-rezaieh and a new cultivar--koli-e-mahallat (syn. escr). the aim of the study was to identify changes in qualitative and q...
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