The standard treatment of human visceral leishmaniasis involves the use of pentavalent antimony (Sb(V)). Its mechanism of action is unknown because of the limited information available about intracellular antimony metabolism and about the genes that regulate these processes. Herein, flow injection-inductively coupled plasma mass spectrometry (ICP-MS), flow injection hydride generation ICP-MS, a...

background: different methods have been used for characterization of leishmania promastigotes. monoclonal antibodies are useful in characterization of   leishmania spp . both amastogotes and promastigotes. objective: comparing the characterization of   leishmania spp. promastigotes with immunoperoxidase test (avidin-biotin) techniques and an indirect immunofluorescent assay (ifa).   methods: ap...

objective(s):cutaneous leishmaniasis is a common and endemic disease in khorasan province in north-east of iran. the pentavalant antimony (sb v) is the mainstay of treatment that has many side effects and resistance to the drug has been reported. the microbicidal effect of ozone was proven in different microorganisms. since there is no study in this respect and to achieve a low cost and effecti...

Objective: To investigate the biochemical capacity, and in vitro inhibitory effects of hairy roots from two cultivars Ficus carica L. (Sabz Siah) on Leishmania major promastigotes amastigotes.Methods: In roots, activity antioxidant enzymes compared to normal leaves presence some phenolic compounds comparison with fruits were investigated. The IC50 values was determined by tetrazolium-dye 3-(4, ...

Mimicking mammalian apoptotic cells by exposing phosphatidylserine (PS) is a strategy used by virus and parasitic protozoa to escape host protective inflammatory responses. With Leishmania amazonensis (La), apoptotic mimicry is a prerogative of the intramacrophagic amastigote form of the parasite and is modulated by the host. Now we show that differently from what happens with amastigotes, prom...

objective(s):solanum acueastrum dunal.has been shown to have some chemotherapeutic value. leaf and berry water and methanol compounds of s. acueastrum were evaluated for possible antileishmanial activity in vivo on balb/c mice and in vitro against leishmania major promastigotes, amastigotes and vero cells.  materials and methods: dry s. aculeastrum berry and leaf material were extracted in meth...

objective: cutaneous leishmaniasis is an endemic infectious disease considered to be a crucial health problem in many countries, including iran. as such, there is a need for new medications with few side effects. in the present research we have studied the effect of artimisinin on  leishmania major (l. major) and cell death in vitro. methods: a specific number of promastigotes of l. major were ...

ABSTRACT        Background and Objective: Leishmania major is a flagellate protozoan parasite causing cutaneous leishmaniasis. Although pentavalent antimony compounds are the first-line drugs for leishmaniasis, their application is often accompanied by numerous limitations and side effects. Therefore, it is necessary to seek drugs of herbal origin that have fa...

Several studies indicate that the outcome of experimental murine cutaneous leishmaniasis caused by Leishmania major (Lm) is determined by immunological events occurring shortly after infection. These events lead to outgrowth of either protective CD4+ T cells in the C57BL/6 mouse, which cures, or exacerbative cells in the BALB/c mouse, which succumbs to disease. Potential factors influencing the...