......................................................................................... 9 INTRODUCTION .................................................................................... 10 Perception of the pathogen ................................................................. 11 Gene-for-gene interactions ........................................................ 11 General elicitors ......

Agrobacterium rhizogenes and Agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring DNA fragments [transfer DNA (T-DNA)] bearing functional genes into the host plant genome. This naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their u...

The evaluation of potato germplasm for resistance to potato tuberworm, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), is a valuable component of integrated pest management; however, few attempts have been made to identify natural genetic tuber resistance to tuberworm on potato germplasm. The objective of this study was to screen potato germplasm with potential tuberworm resistance...

Steroidal glycoalkaloids (SGA) are toxic secondary metabolites present in some members of the Solanaceae family, including potato and tomato. The SGA level in tubers of potato (Solanum tuberosum L.) depends on genetic factors, but can also increase in response to e.g. wounding and light exposure. An upper limit of 200 mg SGA/kg f.w. is recommended in tubers used for human consumption. The SGA b...

Different mutants of an infectious full-length clone (p35PPV-NAT) of Plum pox virus (PPV) were constructed: three mutants with mutations of the assembly motifs RQ and DF in the coat protein gene (CP) and two CP chimeras with exchanges in the CP core region of Zucchini yellow mosaic virus and Potato virus Y. The assembly mutants were restricted to single infected cells, whereas the PPV chimeras ...

• Potato (Solanum tuberosum) calcium-dependent protein kinase (StCDPK5) has been shown to phosphorylate the N-terminal region of plasma membrane RBOH (respiratory burst oxidase homolog) proteins, and participate in StRBOHB-mediated reactive oxygen species (ROS) burst. The constitutively active form, StCDPK5VK, provides a useful tool for gain-of-function analysis of RBOH in defense responses. • ...

Myzus persicae is a devastating pest affecting potato production. Intron-containing hairpin RNA (ihpRNA) silenced the odorant-binding protein 8 (OBP8) for enhanced protection against in potatoes. OBP8 was isolated from M. persicae, sequenced, with allotment of GenBank ID. ERNAi used to design siRNA targets no off-targets. Multiple Sequence Alignment show resemblance Acyrthosiphon pisum, Rhopalo...

Genetically modified plants (GMPs) proved to be a promising solution for many of biotic and abiotic stresses which affect food production. Great attention was given to the evaluation of growth, nutritional quality and safety of GMPs before release and commercialization. In this study, we targeted physiological and biochemical evaluation of transgenic potato plantlets under salt stress condition...

Oxidative stress is a major threat for plants exposed to various environmental stresses. Previous studies found that transgenic potato plants expressing both copper zinc superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) (referred to as SSA plants), or nucleoside diphosphate kinase 2 (NDPK2) (SN plants), showed enhanced tolerance to methyl viologen (MV)-induced oxidative stress and h...

The aim of this work was to investigate the effect of decreased cytosolic pyruvate kinase (PKc) on potato (Solanum tuberosum) tuber metabolism. Transgenic potato plants with strongly reduced levels of PKc were generated by RNA interference gene silencing under the control of a tuber-specific promoter. Metabolite profiling showed that decreased PKc activity led to a decrease in the levels of pyr...