نتایج جستجو برای: polyhydroxybutyrate phb

تعداد نتایج: 1668  

Journal: :Marine science and technology bulletin 2021

Polyhydroxybutyrate (PHB) is the most widely studied biodegradable plastic that does not release any toxins or residues in environment like petroleum based plastics. This work has been undertaken to screen PHB accumulating microorganisms from marine sponges and a total of sixteen isolates were collected purified. Screening isolated strains was done by Nile blue staining observed under Leica LSC...

Journal: :Applied and environmental microbiology 2010
Tina Hölscher Uta Breuer Lorenz Adrian Hauke Harms Thomas Maskow

In this study, a methylotrophic bacterium, Methylobacterium rhodesianum MB 126, was used for the production of the chiral compound (R)-3-hydroxybutyrate (R-3HB) from methanol. R-3HB is formed during intracellular degradation of the storage polymer (R)-3-polyhydroxybutyrate (PHB). Since the monomer R-3HB does not accumulate under natural conditions, M. rhodesianum was genetically modified. The g...

2014
Cha Ping Liau Mansor Bin Ahmad Kamyar Shameli Wan Md Zin Wan Yunus Nor Azowa Ibrahim Norhazlin Zainuddin Yoon Yee Then

Polyhydroxybutyrate (PHB)/polycaprolactone (PCL)/stearate Mg-Al layered double hydroxide (LDH) nanocomposites were prepared via solution casting intercalation method. Coprecipitation method was used to prepare the anionic clay Mg-Al LDH from nitrate salt solution. Modification of nitrate anions by stearate anions between the LDH layers via ion exchange reaction. FTIR spectra showed the presence...

Journal: :Journal of bacteriology 1998
H Matsusaki S Manji K Taguchi M Kato T Fukui Y Doi

Two types of polyhydroxyalkanoate (PHA) biosynthesis gene loci (phb and pha) of Pseudomonas sp. strain 61-3, which produces a blend of poly(3-hydroxybutyrate) [P(3HB)] homopolymer and a random copolymer (poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) [P(3HB-co-3HA]) consisting of 3HA units of 4 to 12 carbon atoms, were cloned and analyzed at the molecular level. In the phb locus, three open read...

Batool Hashemibeni, Reza Samanipour, Saeed Karbasi,

Introduction: As the ability to repair cartilage tissue in body is limited, finding a suitable method for cartilage regeneration has gained the attention of many scholars. For this purpose, scaffold structure and morphology, along with cell culture on it, can be a novel method to treat cartilage injuries, osteoarthritis. Methods: In this study, polyhydroxybutyrate (PHB) is selected as the scaf...

Journal: :Biochemistry 2000
Y Jia T J Kappock T Frick A J Sinskey J Stubbe

Polyhydroxybutyrate (PHB) synthases catalyze the conversion of beta-hydroxybutyryl coenzyme A (HBCoA) to PHB. These enzymes require an active site cysteine nucleophile for covalent catalysis. A protein BLASTp search using the Class III Chromatium vinosum synthase sequence reveals high homology to prokaryotic lipases whose crystal structures are known. The homology is very convincing in the alph...

2015
Janice S. Chen Brendan Colón Brendon Dusel Marika Ziesack Jeffrey C. Way Joseph P. Torella Guo-Qiang Chen

Ralstonia eutropha H16 is a facultatively autotrophic hydrogen-oxidizing bacterium capable of producing polyhydroxybutyrate (PHB)-based bioplastics. As PHB's physical properties may be improved by incorporation of medium-chain-length fatty acids (MCFAs), and MCFAs are valuable on their own as fuel and chemical intermediates, we engineered R. eutropha for MCFA production. Expression of UcFatB2, ...

Journal: :Biochemistry 2005
Jiamin Tian Anthony J Sinskey JoAnne Stubbe

Polyhydroxybutyrate (PHB) synthase catalyzes the polymerization of (R)-3-hydroxybutyryl-CoA (CoA = coenzyme A) into high molecular weight PHB. Recombinant wild-type (wt) class III synthase from Allochromatium vinosum (PhaCPhaE(Av)), antibodies to this synthase and to PHB, and [(14)C]hydroxybutyryl-CoA (HB-CoA) have been used to detect oligomeric hydroxybutyrate (HB) units covalently bound to th...

Journal: :Biomedizinische Technik. Biomedical engineering 2010
Tomasz Gredes Christiane Kunert-Keil Marzena Dominiak Tomasz Gedrange Magdalena Wróbel-Kwiatkowska Jan Szopa

In many studies, natural flax fibers have been proven to be resistant and surgically suitable. Genetically modified flax fibers, derived from transgenic flax expressing three bacterial genes for the synthesis of poly-3-hydroxybutyric acid (PHB), have better mechanical properties than unmodified flax fibers. The aim of this study was to examine the biocompatibility of composites containing flax ...

2011
Hiromichi Hoshina Yusuke Morisawa Harumi Sato Hiroaki Minamide Isao Noda Yukihiro Ozaki Chiko Otani

Temperature-dependent terahertz (THz) absorption spectra of poly(3-hydroxyalkanoate)s (PHAs) were measured by using a Fourier transform far-infrared (FT-FIR) spectrometer and a THz time-domain spectrometer over a temperature range of 10 K to 465 K with a liquid helium cryostat and a heating cell. Clear differences were observed between the spectra of crystalline and amorphous polyhydroxybutyrat...

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