Eosinophil granule proteins are deposited in cutaneous lesions in many human diseases, but how these proteins contribute to pathophysiology is obscure. We injected eosinophil cationic protein (ECP or RNase 3), eosinophil-derived neurotoxin (EDN or RNase 2), eosinophil peroxidase (EPO), and major basic protein-1 (MBP1) intradermally into guinea pig and rabbit skin. ECP and EDN each induced disti...

The effect of storage temperature on the viability of Trichophyton mentagrophytes in infected guinea pig skin scales was quantitatively evaluated for 182 days. Infected skin scales were stored, while protected from light, in glass containers with desiccant at four temperatures and sampled after saline treatment at various time intervals. No decrease in viability was observed for storage at 4 de...

Hypertrophic scarring is a major postoperative complication which leads to severe disfigurement and dysfunction in patients and usually requires multiple surgical revisions due to its high recurrence rates. Excessive-mechanical-loading across wounds is an important initiator of hypertrophic scarring formation. In this study, we demonstrate that intradermal administration of a single extracellul...

This paper describes the first methodology and results for estimating the order of probability for Tasers directly causing human ventricular fibrillation (VF). The probability of an X26 Taser causing human VF was estimated using: (1) current density near the human heart estimated by using 3D finite-element (FE) models; (2) prior data of the maximum dart-to-heart distances that caused VF in pigs...

Protein tyrosine kinase blockers of the tyrphostin family inhibited the EGF-dependent proliferation of human and guinea pig keratinocytes grown in culture and induced their growth arrest. These blockers also significantly inhibited the growth of epidermal keratinocytes, but not of dermal cells, in whole skin organ culture from both guinea pig and human origin. The antiproliferative activity of ...

The commercially available antibodies for immunohistochemical purposes, though numerous and diversified, are mostly designed to detect human antigens. This study was undertaken to evaluate the usefulness of anti-human antigen antibodies in guinea pig tissues. The subjects of our interest were mostly skin and striated muscles and it was important for us to stain vascular and neural elements and ...

  Background: As increased outdoor activities of people, they are more exposed to UV radiation, which causes harmful effects on skin. Silymarin has protective potentials against DNA damage and non-melanoma skin cancers, and also it has anti-oxidant, anti-inflammatory, and immune regulation characteristics. Nano formulation of plant material showed to be more effective than common ones.   Object...

1. The OECD Test Guideline Programme periodically reviews progress in test method development and refinement, both in terms of scientific advances and animal welfare, to determine whether existing Test Guidelines should be updated and whether new Guidelines should be developed. Toward that end, a new assay for the determination of skin sensitisation in the mouse, the Local Lymph Node Assay (LLN...