A relatively simple, very sensitive bioluminescence-enhanced detection system for protein blots is described. The method utilizes antibodies conjugated with alkaline phosphatase. The alkaline phosphatase then takes part in a reaction by releasing D-luciferin (Photinus pyralis) from D-luciferin-O-phosphate. Liberated D-luciferin reacts with luciferase, ATP and oxygen with light emission. The lig...

We are just a few years away from celebrating the 200 anniversary of photography. The first permanent photographic record was made by Niépce in 1826, the view from his window at Le Gras. After many development cycles, including some periods of stagnation, photography is now experiencing an amazing period of growth. The changes that started in the mid 90’s and going into the next several years w...

Ophthalmic imaging has changed dramatically since the 1960s with increasingly complex technologies now available. Arguably, the greatest changes have been the development of the digital camera and the speed, processing power and storage of electronic data. Already, ophthalmic practices in many major institutions overseas have paperless medium storage and electronically generated reporting from ...

In 2017 the Swedish government launched a new Digital Strategy, with overall goal for Sweden to be best in world use of digitalization opportunities. Museums, archives and libraries are important organizations when it comes fulfilling that goal. The interest museum collections is increasing that, need explore increases. Centre Conservation Cultural Property Kiruna part national Sweden’s ...

This report describes a simple technique for the production and use of Formvar film replicas of rulings, such as counting grids, etched on glass slides. The resulting thin, transparent films, when dried on cover slips, are an excellent tool for counting and for orientation in slide cultures (Taubeneck, 1957). The method is similar to that of Powell (1956) who employs a lattice-like ruled area o...

Certain phenol derivatives, including p-iodophenol and p-phenylphenol, enhance light emission from the horseradish peroxidase-catalyzed oxidation of cyclic diacyl hydrazides such as luminol. The light emission decays slowly (glowing for several minutes) and its intensity may be greater than 1000-fold that of the unenhanced reaction. The enhanced system enables rapid, sensitive assay of peroxida...

An x-ray focusing crystal von Hamos spectrometer was developed using mica and graphite cylindrical crystals (radius of curvature R=20 mm) with a CCD linear array detector. The commercial CCD (Toshiba model TCD 1304AP) used for x-ray detection has 3724 pixels (8 μm width and 200 μm height) giving a total length of ~30 mm. The CCD detector was absolutely calibrated using laser-produced plasmas. T...

Three different techniques were used to determine the Modulation Transfer Function (MTF) of a 35mm film scanner. The first involved scanning sine wave charts comprising a number of patches with different frequencies of known modulation. The second method involved the scanning and Fourier transform of a photographic grain noise pattern to simulate low modulation white noise. Finally, the ISO 122...

I detail a method for producing high quality Fourier transform holograms using a computer, laser printer, and overhead transparency film. The method should be useful for lecture demonstration and holography laboratories. Given an arbitrary holographic phase/amplitude plate, computer-generated holography attempts to find a suitable binary encoding of the (complex) transmission function of the ho...