We developed a rapid PCR method utilizing the diversity of the insertion site IS1203 for genotyping Shiga toxin-producing Escherichia coli (STEC) O157 (IS1203 PCR typing). DNA fragments digested by PvuII, which cut IS1203 at one site, were ligated with themselves and detected by PCR with outward-facing primer pairs for IS1203. To minimize nonspecific bands, nested PCR was also performed. Two fi...

The work involved a molecular biological technique (ISSR-PCR) using ISSR-primer S-2, with structure (AGC) 6G. Changes in the gastric mucosa chronic duodenal ulcer disease against background of severe atrophic gastritis have been analyzed. Noteworthy is fact that there strong correlation between degree dysplasia epithelium and mitotic index, Pearson's coefficient rxy was 0.853, respectively. A v...

Brazilian dairy Gir (Bos indicus) cattle are a tropical, well-adapted breed, for which no information on the major histocompatibility complex (MHC) is presently available. The second exon of the bovine lymphocyte antigen (BoLA-DRB3) was amplified by polymerase chain reaction (PCR) of DNA samples from 28 Brazilian dairy Gir cows. Two experimental genotyping approaches were used: direct sequencin...

Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule hasbeen implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction(PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified afragment of 1044 bp from all of tested isolates. It was found that all avian P. mul...

Background: Trichophyton rubrum (T. rubrum) is the most common cause of dermatophytosis of skin and nail tissue. Strain identification in Trichophyton rubrum is important for identification of strain-related differences in infectivity potential or transmissibility and epidemiological studies. PCR typing could determine whether the original isolate is responsible for re-infection or a new strain...

The importance of assays for the detection and typing of human papillomaviruses (HPVs) in clinical and epidemiological studies has been well demonstrated. Several accurate methods for HPV detection and typing have been developed. However, comparative studies showed that several assays have different sensitivities for the detection of specific HPV types, particularly in the case of multiple infe...

DNA methods have resulted in improved renal allograft survival rates in cadaveric renal transplantation. This paper describes the impact of DNA typing by PCRSSP on a living related renal transplant (LRRT) programme. It evaluates error rates in serology, acute rejections, graft function and survival rates between the two typing methods. Serological typing was done on CTS 120 antisera Class 1 and...

BACKGROUND The genetic diversity of Mycoplasma agalactiae (MA) isolates collected in Spain from goats in an area with contagious agalactia (CA) was assessed using a set of validated and new molecular typing methods. Validated methods included pulsed field gel electrophoresis (PFGE), variable number of tandem repeats (VNTR) typing, and Southern blot hybridization using a set of MA DNA probes, in...