Electrophoresis is the main metod for separating large polyelectrolyte molecules, primarily used on DNA, in biochemistry and medicine research. An overwiev of the models of physical mechanisms that are behind this phenomena will be presented. The prominent role is taken by reptation mechanisms, by which large ‡exible polyelectrolytes thread their way through the pores of the gel matrix. There a...

The separation of polypeptides by electrophoresis allowed Arne Tiselius to describe protein fractions corresponding to albumin, -, -, and -globulins in serum with the Rrst published diagram of human serum protein electrophoresis in 1939. The number of fractions slowly expanded into electrophoretic subfractions identiRed as 1, 2, 1, 2, 1 and 2. The mobility characteristics of these fractions are...

Aluminosilicate gel precursor having mullite composition was synthesized from inorganic salts of aluminum and silicon by employing the sol-gel method. Chemical analysis, surface area and bulk density measurements were performed to characterize the dried gel. The course of the mullitization was examined by FT-IR analysis which confirmed the diphasic nature of the gel. ...

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

Lallemantia royleana (Balangu) is a well known Iranian medicinal plant that its seed mucilage has many applications in modern pharmacology. Plant mucilage traditionally was used as a gel supplement, and natural matrix for sustained release of drugs. But it seems that these compounds are not a simple additive and also have many undiscovered pharmacological properties. In this research, the anest...

aloe vera is a plant of the liliaceae family with a brilliant history in disease treatment. in addition to the pharmaceutical, cosmetic, and health care industries, this plant is used in food industry due to having various nutrients such as amino acids and various vitamins or anti-bacterial compounds. the purpose of this study was to investigate the effects of variables such as temperature, tim...

background: in this study the basic radiation properties of n-isopropylacrylamaide polymer gel dosimeter were determined together with verification of its soft tissue equivalency. materials and methods: the nipam gel was prepared and irradiated approximately 2 h after manufacturing. the magnetic resonance (mr) images were made 24 h after irradiation. the nuclear magnetic resonance (nmr) respons...

materials and methods in this study, 90 patients with second-degree burn injuries who were admitted to the yazd burn hospital were randomly divided into three equal groups. in the negative control group, the wounds were covered with sterile vaseline gauze followed by double sterile dry gauze and ultimately bandaged. in the test group, the wounds were covered by an iranian hydrogel sheet (irgel)...

Seven buffer and gel systems for polyacrylamide gel electrophoresis using discontinuous voltage and pH gradients (disc electrophoresis according to ORNSTEIN-UAVIS) and five systems using discontinuous voltage gradients at constant pH (according to ALLEN) have been compiled and characterized. Usefulness and applicability of the systems for clinical investigations are discussed and illustrated by...