The extraction rates of cadmium(II) and zinc(II) with dithizone (H2dz) in the presence of nitrilotriacetic acid (NTA) were measured, and the possible kinetic separation of cadmium(II) from zinc(II) was investigated. Upon the addition of NTA, the difference in the extraction rate between cadmium(II) and zinc(II) became large. Based on the observed rate constant under the condition [NTA] = 1 x 10...

Reactivities of l-'r" chelates of aminopolycarboxylic acids with DNA were investigated by the DNA-sequencing technique using 32P 5'-endlabeled DNA fragments obtained from the human c-Ha-ras-1 protoon cogene, and the reaction mechanism was studied by electron spin reso nance spectroscopy. Ferric nitrilotriacetate (Fe3*-NTA) plus hydrogen peroxide caused strong DNA cleavage in the presence of alb...

Recently, a new and versatile assay to determine the partitioning coefficient [Formula: see text] as a measure for the affinity of peripheral membrane proteins for lipid bilayers was presented in the research article entitled, "Introducing a fluorescence-based standard to quantify protein partitioning into membranes" [1]. Here, the well-characterized binding of hexahistidine-tag (His6) to NTA(N...

A new analytical system is described which provides total particle count and high-resolution size measurements for biological structures such as virus and protein aggregates. This system builds on the Nanoparticle Tracking and Analysis (NTA) technique for the simultaneous visualization and individual sizing of nanoparticles based on their Brownian motion. Virus production and purification proce...

Herein the problem of colloidal instability on electrochemically detected nanoparticle (NP) collisions with a Hg ultramicroelectrode (UME) by electrocatalytic amplification is addressed. NP tracking analysis (NTA) shows that rapid aggregation occurs in solution after diluting citrate-stabilized Pt NPs with hydrazine/phosphate buffers of net ionic strength greater than 70 mM. Colloidal stability...

Relativistic density functional calculations were applied to study the separation behaviors of the Am(iii) ion from the Eu(iii) ion by diglycolamide (DGA) and nitrilotriacetamide (NTA) ligands in order to understand the difference in the separation mechanism of their reagents. The complexation reaction was modeled on the basis of previous experimental studies. The calculated energies based on s...

RAF kinases are key players in the MAPK signaling pathway and are important targets for personalized cancer therapy. RAF dimerization is part of the physiological activation mechanism, together with phosphorylation, and is known to convey resistance to RAF inhibitors. Herein, molecular dynamics simulations are used to show that phosphorylation of a key N-terminal acidic (NtA) motif facilitates ...

The German philosopher K.C.F. Krause (1781–1832) found deep conceptual parallels between his panentheistic system and the Indian philosophy of Ved?nta. This article critically examines Krause’s understanding Ved?nta popular Hindu religion. I argue that while was correct in viewing mystical doctrine as a precursor to own philosophy, he also frequently misled by unreliable translations secondary ...

The analytical technique DGT (Diffusive Gradients in Thin-films) is able to gain access a wealth of information by carefully interpreting accumulation data from passive samplers with different configurations (i.e. thicknesses its constituent layers). A set devices were simultaneously deployed solutions Ni and nitrilotriacetic acid (NTA) concentrations measure the availability these solutions. A...

Energy-resolved threshold collision-induced dissociation (TCID) of negatively-charged [amb5-H+M(II)+NTA-2H]− ternary complexes were studied for how they competitively dissociated into the products [amb5-3H+M(II)]− + NTA or [NTA-3H+M(II)]− amb5, where M = Zn Ni and is nitrilotriacetic acid. The contained one alternative metal binding-5 (amb5) heptapeptides with primary structures acetyl-Aa1-Aa2-...