Background Good sleep quality is associated with a diverse range of positive outcomes such as better health, less daytime sleepiness, well-being, and proper psychological functioning. Sleep deprivation or poor leads to many metabolic, endocrine, immune changes. Many studies have shown changes in schedule along worsening during the COVID-19 pandemic.</n...

Background: Prior to undertaking a study looking at the effects of COVID-19 pandemic upon lived experiences hospice services in West Midlands, we sought identify range issues that service users and providers faced between March 2020 July 2021, provide report can be accessed understood by all interested stakeholders. M...

In addition to NS3 protease, the NS4A protein is required for efficient cleavage of the nonstructural protein region of the hepatitis C virus polyprotein. To investigate the function and the sequence of NS4A required for the enhancement of NS3 protease activity, we developed an in vitro NS3 protease assay system consisting of three purified viral elements: (i) a recombinant NS3 protease which w...

Background: To date, coronavirus diseases 2019 (COVID-19) has no definitive treatment. Thrombosis and hypercoagulation may occur in the advanced stage. Further study on how to use anticoagulants is still required promote best prognosis. Methods: A cross-sectional of 110...

Household food insecurity and poor well-being have increased during the coronavirus disease 2019 (COVID-19) pandemic resulting lockdown measures. Home growing has been associated with improved access well-being, but it is unknown what role plays supply crises lockdown. It also unclear how home social restrictions may affect opinions about in urban areas (i.e., agriculture [UA]).<...

Introduction: Indonesia has the second highest number of Tuberculosis (TB) in world and tuberculosis still pose as a global health priority problem. For diagnostics, microscopy is one important modalities TB diagnosis especially peripheral areas for screening, case-finding, treatment evaluation. The use limited to operator visual burden specimen ...

The NS3 protein of Japanese encephalitis virus (JEV) contains motifs typical of RNA helicase/NTPase but no RNA helicase activity has been reported for this protein. To identify and characterize the RNA helicase activity of JEV NS3, a truncated form of the protein with a His-tag was expressed in Escherichia coli and purified. The purified JEV NS3 protein showed an RNA helicase activity, which wa...

Backgroundː Numerous in vivo human cohort studies have suggested that the apolipoprotein B100/apolipoprotein AI (ApoB100/ApoAI) ratio might be a risk factor in coronary heart disease. The aim of this study was to measure ApoB100/ApoAI changes cell secretions by incubating HepG2 cells with various amounts glucose vitro.<ns...

Infections with Hepatitis C virus (HCV) pose a serious health problem worldwide. In this study, the hypothesis that adoptive transfer of dendritic cells (DCs) pulsed with HCV NS3 protein and matured with an oligodeoxynucleotide (ODN) containing CpG motifs (CpG) ex vivo would initiate potent HCV-specific protective immune responses in vivo was tested. NS3 protein was efficiently transduced into ...