Magnetic-fluorescent nanoparticles can offer various applications such as imaging, labeling, separation, and therapy. Here we have functionalized magnetic nanoparticle (MNP) with fluorescent dyes such as acridine orange (ACO) and rhodamine-B (RHB) via chemical crosslinkers. The fluorescent MNPs were characterized by TEM, SQUID, and FTIR to prove the successful conjugation. Fluorescent spectrosc...

Agilent’s dual-mode Gene Expression Microarray platform is a comprehensive and robust system that couples the ease and simplicity of one-color experimental design with the capability of two-color sensitivity for detection of small gene expression changes—all in a single platform. The Agilent platform now includes a method for the analysis of differential gene expression from human whole blood, ...

Objective: Tumor establishment, metastatic spreading and poor survival in ovarian cancer is strongly associated with progressive derangement of the patient’s immune system. Accumulating evidence suggests that immune impairment is influenced by the production and presence of cytokines in the tumor microenvironment. Methods: Cytokine mRNA profiles in tumor tissue and peripheral blood mononuclear ...

Protein purification is essential for advancements in biotechnology. There are several different methods employed in purifying a particular protein from a complex sample such as a cell lysate. These methods take advantage of differences in the size, charge or binding affinity of the protein. One such method is affinity chromatography which utilizes the binding affinity of a protein toward a cer...

Despite the increasing clinical importance of nonalcoholic fatty liver disease (NAFLD), little is known about its underlying pathogenesis or specific treatment. The senescence marker protein 30 (SMP30), which regulates biosynthesis vitamin C (VC) in many mammals, except primates and humans, was recently recognized as a gluconolactonase. However, precise relation between VC lipid metabolism NAFL...

Cucumber mosaic virus (CMV) is a cosmopolitan virus which may also have small satellite RNAs (satRNA) associated with it affecting symptom development. Vinca minor (periwinkle) plants exhibiting subtle mosaic symptoms tested positive for CMV by enzyme linked immunosorbent assay (ELISA). Double-stranded ribonucleic acid (dsRNA) analysis of CMV-Vinca field isolates in Nicotiana tabacum ‘Glurk’ su...

The subject of bacterial resistance to antibiotics has not only inundated the commercial market, but also provided powerful evolutionary insight into larger studies of evolution. Genetic resistance using bacterial models is highly regarded for their rapid proliferation, easy maintenance, and simple manipulation of variables. Despite their widespread use, these studies have yet to signify the pr...

The people have different behaviour to choose the food, and there are many factors that affect the food choices. The best significant factor to choose the food is taste. Differences in taste perception of several taste modalities are associated to difference in the taste receptors. Polymorphisms of the genes that encoding these taste receptors may clarify this unpredictability in taste percepti...

Prostate cancer is the most frequently diagnosed non-cutaneous cancer and the second leading cause of cancer-related deaths in American men. Recent studies have suggested that sarcoma inducing kinase (SRC) and its downstream signaling targets are implicated in prostate cancer, but the mechanisms behind how SRC inhibition induces apoptosis are still poorly understood. This study focused specific...

Phalaenopsis is the second most valuable potted plant in the United States. Information on the genetic diversity and relationships among species and hybrids is important for breeding purposes and species conservation. In this study, genetic variability of 16 Phalaenopsis species and hybrids was analyzed using amplified fragment length polymorphism (AFLP) markers. Ten AFLP primer combinations am...