Introduction. Increased resistance of the heart to ischemia/reperfusion (I/R) is an urgent aim physiology, pharmacology, and cardiac surgery, since I/R injury often cause cardiogenic shock subsequent death patients in postoperative period. Materials methods. The study was carried out male rats which were subjected coronary artery occlusion (45 min) reperfusion (2 h). Before occlusion, early hyp...

This study aimed to determine the impact of limited face-to-face class intervention on achievements students in English 7. A Post-Test Only Non-equivalence Groups Design was used. total twenty-four (24) respondents were conveniently chosen wherein twelve (12) these attended using modules and other exposed modular print only. The Mann Whitney U test result (U = 114: p .01581), indicates that Lim...

We present high angular resolution (∼ 1 and 0.6) mm-interferometric observations of the CO(1− 0) and CO(2− 1) line emission in the central 300 pc of the late-type spiral galaxy NGC6946. The data, obtained with the IRAM Plateau de Bure Interferometer (PdBI), allow the first detection of a molecular gas spiral in the inner ∼ 10 (270 pc) with a large concentration of molecular Based on observation...

Overexpression of N-methylpurine DNA glycosylase (MPG) has been suggested as a possible gene therapy approach to sensitize tumor cells to the cell-killing effects of temozolomide, an imidazotetrazine-class chemotherapeutic alkylating agent. In the present study, we show that both elevated MPG expression and short hairpin RNA-mediated loss of DNA polymerase (Pol ) expression in human breast canc...

Autophagy is a bulk degradation process in which cytosolic proteins and organelles are degraded through lysosomes. To evaluate autophagic flux in cardiac myocytes, we generated adenovirus and cardiac-specific transgenic mice harboring tandem fluorescent mRFP-GFP-LC3. Starvation significantly increased the number of mRFP-GFP-LC3 dots representing both autophagosomes and autolysosomes per cell, s...

Previous results from our laboratory showed that phosphorylation of ryanodine receptor 2 (RyR2) by Ca(2+) calmodulin-dependent kinase II (CaMKII) was a critical but not the unique event responsible for the production of reperfusion-induced arrhythmogenesis, suggesting the existence of other mechanisms cooperating in an additive way to produce these rhythm alterations. Oxidative stress is a prom...

Clusters composed of a 32 silver atom core, protected with thiolates of glutathione (GSH) and N-(2-mercaptopropionyl)glycine (MPGH), were synthesized by a solid-state route in milligram scale. They do not exhibit surface plasmon resonance unlike their larger sized nanoparticle analogues but show molecule-like features in absorption and luminescence spectra, falling in the visible window. The co...

A report of the meeting “Challenges in experimental data integration within genomescale metabolic models”, Institut Henri Poincaré, Paris, October 10-11 2009, organized by the CNRS-MPG joint program in Systems Biology.

The development of short interfering RNA (siRNA), has provided great hope for therapeutic targeting of specific genes responsible for pathological disorders. However, the poor cellular uptake and bioavailability of siRNA remain a major obstacle to their clinical development and most strategies that propose to improve siRNA delivery remain limited for in vivo applications. In this study, we repo...

The DNA damage response (DDR) is activated by various genotoxic stresses. Base lesions, which are structurally simple and predominantly fixed by base excision repair (BER), can trigger the ataxia telangiectasia mutated (ATM)-checkpoint kinase 2 (Chk2) pathway, a DDR component. How these lesions trigger DDR remains unclear. Here we show that, for alkylation damage, methylpurine-DNA glycosylase (...