Electrophysiological signals were recorded from primary cultures of dissociated rat cortical neurons coupled to MicroElectrode Arrays (MEAs). The neuronal discharge patterns may change under varying physiological and pathological conditions. For this reason, we developed a new burst detection method able to identify bursts with peculiar features in different experimental conditions (i.e. sponta...

A micro glucose sensor consisting of an interdigitated array gold microelectrode was developed. The interdigitated array structure, which has 10 microns band width and 10 microns band gap, was fabricated in a small region (2.5 x 5 mm2) on a quartz substrate. Glucose oxidase was chemically fixed onto the electrode surface through self-assembled monolayer of 11-mercaptoundecanoic acid; ferrocenea...

A new design for microelectrode array (MEA) devices fabricated by semiconductor-processing techniques is presented. The microelectrode surfaces consist of gold and are surrounded by an insulating silicon nitride layer. Each chip of these so-called Multi MEAs contains regular arrays with circular-shaped electrodes of eight different sizes: 1, 3, 5, 10, 50, 100, 500 and 1000μm. The Multi MEAs wer...

The application of printed circuit technology to the production of a new type of multi-channel microelectrode is described. 2. Recordings have been obtained from the the protocerebrum of the honeybee Apis mellifera L. using printed circuit microelectrodes in both restrained and free-moving preparations. 3. These recordings are compared with those previously obtained from conventional probe micr...

A novel microelectrode modification method is reported for neural electrode engineering with a flake nanostructure (nanoflake). The nanoflake-modified electrodes are fabricated by combining conventional lithography and electrochemical deposition to implement a microelectrode array (MEA) on a glass substrate. The unique geometrical properties of nanoflake sharp tips and valleys are studied by op...

This paper presents spatially arranged microelectrode probes to allow electrophysiological recordings inside 3–D neuronal cultures. Out–of–plane microelectrode probes standing on a substrate have gradation in height. Wire– bonding–based probe technology [1] makes it possible to provide the flexible probes. We would understand the dependent alteration of cellular activities caused by the constru...

Intended for scientists interested in electrophysiology, this symposium will include methodological talks by international scientists within the field. Speakers from diverse areas will present results illustrating advances in neuroscience made possible by advances in instrumentation. The first half of the symposium will be devoted to microelectrode techniques and the second half will be devoted...

We developed the fibrin gel-based C2C12 myotube culture system integrated with the microelectrode array device to afford a physiologically relevant and stable bioassay system. Myotube/fibrin gel culture system was prepared by transferring a myotube monolayer from a glass substrate to a fibrin gel while retaining the original line patterns of myotubes. By attaching the myotube/fibrin gel onto th...

Lithographically fabricated iridium-based mercury microelectrode arrays are shown to be very suitable for adsorptive stripping measurements of trace nickel in the presence of the dimethylgloxime complexing agent. Variables affecting the response have been assessed and optimized. The sensor optimization has resulted in a favorable and highly stable response, with good linearity and precision (RS...

This paper describes an integrated microelectrode stimulator for intracortical neuroprosthesis. The implantable microelectrode stimulator uses flip-chip technology to be fully implantable without wiring, reducing the risk of infection and increasing robustness. It is small enough to be undetectable and has low power consumption which can be obtained directly from the carrier, through an RF low-...