Genetic engineering now makes possible the insertion of DNA from many organisms into other prokaryotic, eukaryotic and viral hosts. This technology has been used to construct a variety of such genetically engineered microorganisms (GEMs). The possibility of accidental or deliberate release of GEMs into the natural environment has recently raised much public concern. The prospect of deliberate r...

Abstract Reductive dissolution during permafrost thaw releases iron-bound organic carbon to porewaters, rendering previously stable vulnerable microbial decomposition and subsequent release the atmosphere. How mineral iron stability processes influencing vary transitional are poorly understood, yet have important implications for cycling emissions. Here we determine reactive associated content ...

We analyzed the volatile organic compounds (VOCs) emitted from different soils by using the PTR-MS-TOF technique under laboratory conditions and compared them with soil chemical biochemical activities. The emitted VOCs were related to soil microbial biomass, soil respiration and some soil enzyme activities so as to evaluate if size and activity of soil microbial communities influenced the soil ...

It is well known that soil microbial communities reestablish following disturbance, but limited research has been done on how long this takes in eastern reclaimed coal mine soils. The objective of this study was to track the development of total microbial biomass in reclaimed mine soils following coal mining in southwestern Virginia. A chronosequence of sites was established based on locally do...

Soil and plant tissues were used to examine the effect on the release of dissolved organic carbon (DOC) of rinsing over time at two temperatures and under oxic and anoxic conditions in a laboratory incubation. The release of DOC over 60 days of incubation ranged from 0.5 to 189 mg DOC g 1 oven-dry material and was correlated inversely with the degree of decomposition of the material: fresh mapl...

An in vitro procedure was developed to estimate rate and extent of ruminal protein degradation using [15N]H3 to quantify uptake of protein degradation products for microbial protein synthesis. Incubations were conducted for 6 h in stirrer flasks with ruminal inoculum plus buffer, reducing solution, pectin, soluble carbohydrates, and added (15NH4)2SO4. Seven protein concentrates were tested in t...

Despite nutrient-depleted conditions, coral reef waters harbor abundant and diverse microbes; as major agents of microbial mortality, viruses are likely to influence microbial processes in these ecosystems. However, little is known about marine viruses in these rapidly changing ecosystems. Here we examined spatial and short-term temporal variability in marine viral abundance (VA) and viral lyti...

It is often assumed that planted wastewater treatment systems outperform unplanted ones, mainly because plants stimulate belowground microbial population. Yet, fundamental interactions between plants and associated microorganisms remain only partly understood. The aim of our project was to evaluate microbial density and activity associated to the rhizosphere of three plant species. Experimental...

There is a great need to understand the environmental impacts of organic pollutants on soil health. Phthalates are widely used in consumables and can be found extensively. We studied the toxicity of diethyl phthalate (DEP), spiked in a compost plant growth substrate, by means of the acute toxicity Flash test and on the basis of the germination and plant growth of radish seedlings. The response ...