Many enzymatic reactions in biochemistry are far more complex than the celebrated Michaelis-Menten scheme, but the observed turnover rate often obeys the hyperbolic dependence on the substrate concentration, a relation established almost a century ago for the simple Michaelis-Menten mechanism. To resolve the longstanding puzzle, we apply the flux balance method to predict the functional form of...

Many chemical reactions in biological cells occur at very low concentrations of constituent molecules. Thus, transcriptional gene-regulation is often controlled by poorly expressed transcription-factors, such as E.coli lac repressor with few tens of copies. Here we study the effects of inherent concentration fluctuations of substrate-molecules on the seminal Michaelis-Menten scheme of biochemic...

this paper investigates the importance of considering biomass growth and decay for predicting a field scale microbial degradation of btex plume in an unconfined aquifer system in the pump station area of tehran oil refinery (tor), iran. a two-dimensional finite volume model has been presented to predict multi-species reactive transport incorporating physical, chemical and biological processes i...

A comparison is made between conventional Michaelis-Menten kinetics and two models that take into account the duration of the conformational changes that take place at the molecular level during the catalytic cycle of a monomer. The models consider the time that elapses from the moment an enzyme-substrate complex forms until the moment a product molecule is released, as well as the recovery tim...

Quantitative biology relies on the construction of accurate mathematical models, yet the e↵ectiveness of these models is often predicated on making simplifying approximations that allow for direct comparisons with available experimental data. The Michaelis-Menten approximation is widely used in both deterministic and discrete stochastic models of intracellular reaction networks, due to the ubiq...

background: dystocia is one of the important causes of maternal morbidity and mortality in low-income countries. this study wasaimed to determine the diagnostic accuracy of maternal anthropometric measurements as predictors for dystocia in nulliparous women. materials and methods: this prospective cohort study was conducted on 447 nulliparous women who referred to omolbaninhospital. several mat...

Phenoloxidases (EC.1.14.18.1) are implicated in the immunity of insects toward microorganisms, so oxidative enzymes such as phenoloxidases (PO) from 7th instar larvae red palm weevil; Rhynchophorus ferrugineus (Oliv.) was partially isolated to characterize its activity using catechol a substrate. The mitochondrial fraction larval homogenate used enzyme source for partial kinetic studies.Enzyme ...