Mitochondrial dysfunction contributes to the pathophysiology of both acute and chronic neurodegenerative disorders. Quantification of mitochondrial bioenergetic properties generally requires the use of isolated brain mitochondria. However, the involvement of neuronal mitochondrial dysfunction in these disorders is limited by the lack of markers, and therefore isolation procedures, that distingu...

Francisella is a gram-negative bacterial pathogen, which causes tularemia in humans and animals. A crucial step of Francisella infection is its invasion of macrophage cells. Biogenesis of the Francisella-containing phagosome (FCP) is arrested for ~15 min at the endosomal stage, followed by gradual bacterial escape into the cytosol, where the microbe proliferates. The crucial step in pathogenesi...

To better understand molecular mechanisms regulating changes in metabolism, as observed e.g. in diabetes or neuronal disorders, the function of mitochondria needs to be precisely determined. The usual isolation methods such as differential centrifugation result in isolates of highly variable quality and quantity. To fulfill the need of a reproducible isolation method from solid tissues, which i...

Fluoride ___ lycolytic No direct evidence is as Fluoride System yet available concerning the nature of the glycerol Pyruvic acid kinase. Presumably like plant hexokinase (13) it is localized in the mitochondria. The isolation and characterization of the enzymes which participate TCA CYCLE in this scheme will contribute -/Malanate further information to the comparative biochemical aspects of thi...

Substation genetic and molecular information has been obtained after 70 years of intense research with Neurospora which provided foundations for studying mitochondrial activities and biogenesis.[Davies, 2002; Borkovich et al., 2004]. The basic understanding of mitochondrial operation has been enhanced tremendously in recent years by advances in structural biology that span the resolution spectr...

Measurements of mitochondrial respiration in intact cells can help define metabolism and its dysregulation in fields such as cancer, metabolic disease, immunology, and neurodegeneration. Although cells can be offered various substrates in the assay medium, many cell types can oxidize stored pools of energy substrates. A general bioenergetic profile can therefore be obtained using intact cells, ...

pioglitazone (pg) is one of thiazolidinediones used for the treatment of type ii diabetes mellitus. some reports of its hepatotoxicity exist, but the mechanism of its hepatotoxicity is not well known. in the present study, the protective effect of some atp suppliers are investigated against mitochondrial toxicity of pg in isolated rat mitochondria. mitochondrial viability was investigated by mt...