The isoenzyme pattern of LDH does not play a physiological role; except perhaps during fast transitions in energy metabolism. Aging, 3(5), 457-460. Introduction: A paper describing increased brain lactate concentration with age recently caught our attension [1]. The proposed explanation for the increased brain lactate with age was a shift in the brain lactate dehydrogenase (LDH) isoenzyme patte...

Mitochondria mediate diverse cellular functions including energy generation and ROS (reactive oxygen species) production and contribute to signal transduction. Mitochondria are also key regulators of cell viability and play a central role in necrotic and apoptotic cell death pathways induced by cardiac ischaemia/reperfusion injury. PKC (protein kinase C) ε plays a critical role in cardioprotect...

The E and S isoenzymes of horse liver alcohol dehydrogenase differ by 10 amino acid residues, but only the S isoenzyme is active on 3 beta-hydroxysteroids. This functional difference was correlated to the differences in structures of the isoenzymes by characterizing a series of chimeric enzymes, which could represent intermediates in the evolution of catalytic activity. Deletion of Asp-115 from...

Serum gamma-glutamyltransferase (GGT) and alkaline phosphatase (AP) were assayed in 98 consecutive patients with rheumatoid arthritis. Twenty-three patients had increased GGT activities and 45 an increased AP activity. Twelve patients showed an increase in both enzyme activities and AP isoenzyme studies were performed on seven of this group. In three subjects an increase in the bone isoenzyme w...

1. GM,-ganglioside, specifically tritiated in the terminal galactose, was hydrolysed by two forms of 'acid' methylumbelliferyl fl-galactosidase isolated on gel filtration. 2. Identification of GM1-ganglioside ,B-galactosidase activity with the 'acid' methylumbelliferyl ,-galactosidases was based on the following: coincident elution profiles on gel filtration; simultaneous inactivation by heat a...

In this study, oxidative energy metabolism was determined in 5 captive bottlenose dolphins (Tursiops truncatus), and subsequently compared to 6 Thoroughbred riding horses, and 12 lactating Holstein cows in order to assess symmorphosis of each animal species. Plasma metabolites profile, plasma MDH (M) and LDH (L) activities, M/L ratio, and plasma LDH isoenzyme distribution patterns were all exam...

Phophodiesterase (PDE) isoenzyme profiles of human cell preparations and tissues have been analysed by a semiquantitative method using selective PDE inhibitors and activators. Neutrophils, eosinophils and monocytes contain PDE IV exclusively. Lymphocytes, alveolar macrophages and endothelial cells contain PDE IV and PDE III, and in addition, PDE I is measured in macrophages and PDE II in endoth...

entamoeba histolytica is the etiological agent of invasive amoebiasis, the third leading parasitic cause of mortality in the world. our aim was to find a molecular correlation between a glucosephosphate isomerase zymodeme analyses in e. histolytica zymodemes. it was demonstrated that natural and recombinant glucosephosphate isomerase enzymes of e. histolytica comigrated in the starch gel electr...