Comparative metabolic modelling is emerging as a novel field, supported by the development of reliable and standardized approaches for constructing genome-scale metabolic models in high throughput. New software solutions are needed to allow efficient comparative analysis of multiple models in the context of multiple cellular objectives. Here, we present the user-friendly software framework Mult...

The understanding of the control of metabolic flux in plants requires integrated mathematical formulations of gene and protein expression, enzyme kinetics, and developmental biology. Plants have a large number of metabolically active compartments, and non-steady-state conditions are frequently encountered. Consequently steady-state metabolic flux balance and isotopic flux balance modeling appro...

A combination of batch fermentations and genome scale flux balance analysis were used to identify and quantify the rate limiting reactions in the xylulose transport and utilization pathway. Xylulose phosphorylation by xylulokinase was identified as limiting in wild type Saccharomyces cerevisiae, but transport became limiting when xylulokinase was upregulated. Further experiments showed xylulose...

Genome-based Flux Balance Analysis (FBA) and steady-state isotopic-labeling-based Metabolic Flux Analysis (MFA) are complimentary approaches to predicting andmeasuring the operation and regulation ofmetabolic networks. Here, genome-derivedmodels of Escherichia coli (E. coli)metabolismwere used for FBA and C-MFA analyses of aerobic and anaerobic growths of wild-type E. coli (K-12 MG1655) cells. ...

Genome-based Flux Balance Analysis (FBA) and steady-state isotopic-labeling-based Metabolic Flux Analysis (MFA) are complimentary approaches to predicting and measuring the operation and regulation of metabolic networks. Here, genome-derived models of Escherichia coli (E. coli) metabolism were used for FBA and ¹³C-MFA analyses of aerobic and anaerobic growths of wild-type E. coli (K-12 MG1655) ...

Metabolic networks can be turned into kinetic models in a predefined steady state by sampling the reaction elasticities in this state. Elasticities for many reversible rate laws can be computed from the reaction Gibbs free energies, which are determined by the state, and from physically unconstrained saturation values. Starting from a network structure with allosteric regulation and consistent ...

Transposon mutagenesis, in combination with parallel sequencing, is becoming a powerful tool for en-masse mutant analysis. A probability generating function was used to explain observed miniHimar transposon insertion patterns, and gene essentiality calls were made by transposon insertion frequency analysis (TIFA). TIFA incorporated the observed genome and sequence motif bias of the miniHimar tr...

We present two modifications of the flux balance analysis (FBA) metabolic modeling framework which relax implicit assumptions of the biomass reaction. Our flexible flux balance analysis (flexFBA) objective removes the fixed proportion between reactants, and can therefore produce a subset of biomass reactants. Our time-linked flux balance analysis (tFBA) simulation removes the fixed proportion b...

Visual speech, i.e., video recordings of speakers’ mouths, plays an important role in improving the robustness properties of automatic speech recognition (ASR) against noise. Optimal fusion of audio and video modalities is still one of the major challenges that attracts significant interest in the realm of audiovisual ASR. Recently, turbo decoders (TDs) have been successful in addressing the au...