An open research field in cellular regulation is the assumed crosstalk between RNAs, metabolic enzymes, and metabolites, also known as REM hypothesis. High-throughput assays have produced extensive interactome data with enzymes frequently found hits, but only a few examples been biochemically validated, deficits especially prokaryotes. Therefore, we rationally selected nineteen Escherichia coli...

Immobilization of single antioxidant enzyme systems was frequently studied in the past, however, there is a lack reliable reports on co-immobilization such enzymes. Here, an cascade involving superoxide dismutase (SOD) and horseradish peroxidase (HRP) successfully immobilized titania nanosheets (TNS) by sequential adsorption method using poly(diallyldimethylammonium chloride) (PDADMAC) poly(sty...

The lpsA gene, a late acting gene in the biosynthetic pathway of ergovaline, a suspected causative agent for fescue toxicosis in cattle, has been cloned from Neotyphodium lolii, an endophytic fungus of Lolium perenne. In this study, a similar gene was detected in several strains of endophytic Neotyphodium spp. isolated from grass hosts endogenous to Iran using direct and nested-PCR assays. Exce...

The study of fish digestive biochemistry is essential to understand factors that affect the net efficiency food transformation and growth, therefore aquaculture profitability. aim present was assess activity functional characteristics key enzymes in juveniles greater amberjack ( Seriola dumerili ), as well possible modulation their relative importance by water temperature. For that, a combinati...

Mikania micrantha Kunth (Asteraceae) is a plant traditionally used to reduce the risk of hyperlipidemia and hypertension. There limited information on anti-hyperlipidemic anti-hypertensive effects various M. leaves stem extracts. This study aimed examine in vitro potential different parts (leaves stem) extracts inhibiting hyperlipidemia-related enzymes, i.e., pancreatic lipase (PL), lipoprotein...

Background: The human cytochrome P450 (CYP) CYP3A4 and CYP3A5 enzymes metabolize more than half of marketed drugs. They share high structural substrate similarity are often studied together as CYP3A4/ 5. However, they preferentially different clinically prescribed Moreover, the differential distribution expression levels in both normal diseased tissues can aggravate toxicity induce resistance d...

The cytochrome P450 (CYP) enzymes are of a ubiquitous heme–thiolate proteins family that plays an important role within the biotransformation endogenous molecules and xenobiotics as today’s market is bio-transformed through CYP-mediated metabolism. Major CYP isoforms involved in most reactions mainly CYP3A4 CYP2D6. found extensively human liver gut which this CYP-isoform takes part phase I tran...

Long INterspersed Element-1 (LINE-1 or L1) retrotransposition poses a mutagenic threat to human genomes. Human cells have therefore evolved strategies to regulate L1 retrotransposition. The APOBEC3 (A3) gene family consists of seven enzymes that catalyze deamination of cytidine nucleotides to uridine nucleotides (C-to-U) in single-strand DNA substrates. Among these enzymes, APOBEC3A (A3A) is th...

A method for sensitively monitoring enzyme kinetics and activities by using dual-color fluorescence cross-correlation spectroscopy is described. This universal method enables the development of highly sensitive and precise assays for real-time kinetic analyses of any catalyzed cleavage or addition reaction, where a chemical linkage is formed or cleaved through an enzyme's action between two flu...

Signaling pathways regulate gene expression programs via the modulation of the chromatin structure at different levels, such as by post-translational modifications (PTMs) of histone tails, the exchange of canonical histones with histone variants, and nucleosome eviction. Such regulation requires the binding of signal-sensitive transcription factors (TFs) that recruit chromatin-modifying enzymes...