We have compared two methods for separating the isoenzymes of creatine kinase in serum and measuring their activity: chromatography on diethylaminoethyl-Sephadex A-50, with both continuous and discontinuous gradient elution, and electrophoresis on cellulose acetate. Results by continuous and discontinuous gradient elution correlated well both for tissue extracts and sera. Electrophoresis on cel...

As a part of this study, mathematical model based on two-compartment pharmacokinetic system with general fractional derivatives was developed. Its aim to describe the release BisGMA and TEGDMA monomers from composite core resin over three time periods. For purpose, five Clearfil Photo Core samples were prepared elution measured using high performance liquid chromatography at 1, 7 28 days post-i...

Chromatographic columns packed with commercially available hydrophobic interaction chromatography (HIC) media were found to be able to suppress aggregation and nevertheless had a tendency to promote the structural misfolding resulting in higher soluble protein recovery and lower specific activity than that by dilution when they were used to refold lysozyme, a model protein. Moreover, this misfo...

Nisin is an antimicrobial peptide produced and secreted by several L. lactis strains and is specifically active against Gram-positive bacteria. In previous studies, nisin was purified via cation exchange chromatography at low pH employing a single-step elution using 1 M NaCl. Here, we describe an optimized purification protocol using a five-step NaCl elution to remove contaminants. The obtained...

Concentration dependence of elution volume was determined for Blue Dextran 2000, Dextran 500, Dextran sulphate 500 and bovine serum albumin on columns of Sephadex G-100 equilibrated with sodium phosphate buffer, I 0.1, pH6.8. From the results for Dextran 500, it was shown that a linear relation exists between elution volume and the corresponding osmotic pressure calculated for the same concentr...

Infection by the human hookworm Necator americanus is a leading cause of anemia and disability in the developing countries of Africa, Asia, and the Americas. In order to prevent childhood hookworm disease in resource poor settings, a recombinant vaccine is under development by the Sabin Vaccine Institute and Texas Children's Hospital Center for Vaccine Development, a Product Development Partner...

Ion exchange is a very useful technique to separate protein mixtures under mild conditions, most commonly by applying a shallow gradient of increasing salt concentration as eluent. pH gradient elution is used much less frequently, except in specialist applications such as chromatofocusing, and can require complex buffer systems. However, today’s modern quaternary HPLC systems are ideally suited...

Even though antibiotic eluting bone cements are successfully used in orthopedic practice, adding chemotherapy drugs to bone cement pose a different set of problems. This is mainly due to the toxicity concerns associated with the use of chemotherapy agents. To avoid local or systemic toxicity, the drug elution profile of a particular bone cement/drug concentration need to be determined. The drug...

A modified broth-disk elution method for routine susceptibility testing of anaerobic bacteria was compared with the proposed reference agar dilution method under study by the National Committee on Clinical Laboratory Standards. The broth-disk elution method used a 24-h test incubation period, Schaedler broth medium supplemented with menadione, and GasPak (BBL Microbiology Systems) incubation. R...