The pregnane X receptor (PXR) regulates the metabolism and excretion of xenobiotics and endobiotics by regulating the expression of drug-metabolizing enzymes and transporters. The unique structure of PXR allows it to bind many drugs and drug leads, possibly causing undesired drug-drug interactions. Therefore, it is crucial to evaluate whether chemicals or drugs bind to PXR. Fluorescence-based a...

Abstract Medicinal plants contain phytochemical components of pharmaceutical importance, and Trema orientalis MeOH extracts are believed to have potential antioxidant cytotoxic properties. This investigation explores the phenolic, antioxidant, anticancer property methanol aerial parts T. . The total polyphenol content (TPC) flavonoid contents (TFC) were determined following standard methods. In...

Drug discovery initiatives, aimed at Chagas treatment, have been hampered by the lack of standardized drug screening protocols and the absence of simple pre-clinical assays to evaluate treatment efficacy in animal models. In this study, we used a simple Enzyme Linked Aptamer (ELA) assay to detect T. cruzi biomarker in blood and validate murine drug discovery models of Chagas disease. In two mic...

Abstract Using the starting reactants of Zn(OAc) 2 and 2-mercaptonicotinic acid (H L), we successfully synthesized a fresh Zn( ii ) compound, that is, [Zn 3 (L) ] n (1), under hydrothermal conditions then loaded with atenolol. Hualuronic acid/carboxymethyl chitosan hydrogels atenolol drug metal–organic framework were prepared based on chemical synthesis method. The microscopic morphology was in...

The MTBDRsl assay (Hain Lifescience GmbH, Germany) is a new line probe assay for the detection of extensively drug-resistant tuberculosis (XDR TB). The test simultaneously detects resistance to ethambutol, aminoglycosides/cyclic peptides, and fluoroquinolones through detection of mutations in the relevant genes. The assay format is identical to the MTBDR Hain assay. The assay was evaluated for ...