background: isolation of high quality genomic dna is one of the most important steps in molecular biology studies related to food borne parasites. usually, various protocols are used for different tissues but so far, there is no common, simple and cost benefit procedure for genomic dna extraction of linguatula serrata ( l. serrata .) larvae as a pentastomida endangering food safety. one of the ...

background: nematodes are among the most common and important parasites of man and animal. dna of a single worm can be used for several purposes, such as identification to the species, subspecies, strain and antihelmenthic resistance. dna extraction from a single small worm using traditional methods such as phenol extraction technique faces serious prob lems. methods: dna from 20 single haemonc...

results the mean ages in case and control groups were 60.8 ± 6.8 and 57.5 ± 11.5 years, respectively, with no significant difference (p = 0.09). thirty patients (54.5%) in case and 32 (58.2%) in control groups were male with no significant difference (p = 0.7). cmv-dna was present in 8 (14.5%) of the cases and 2 (4%) of the controls. cmv-dna was associated with higher risk of atherosclerosis (o...

mitochondria are membrane-enclosed organelle found in most eukaryotic cells, which known as power house in cells. this organelle transforms energy into forms that are usable by the cell. the most common psychiatric disorders such as depression and anxiety can be linked to mitochondrial disorders. furthermore, mutations of mitochondrial or nuclear dna (mtdna and ndna, respectively) have been lin...

objective: a recent field of research in epigenetics is dna methylation which involves the cpg island in the genome that subsequently controls transcription and translation of targeted genes. in the hepatitis b virus (hbv) genome, there are three cpg islands which tend to be methylated. the aim of the current study is to determine the methylation pattern of the hbv x gene in chronically infecte...

the internal structure of dna-ctab and dna-ddab is investigated by a small angel x- ray scattering(saxs) instrument. hexagonal packing of dna was observed for dna complex with ctab, and for ddabcomplex is observed lamellar structure. variations in the internal spacing and degree of long-range ordering aredependent on both surfactant type and concentrations of added salt. when we increased the a...

background: hepatitis c virus (hcv) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. therefore, designing new and effective therapeutics is of great importance. the aim of the current study was to construct a dna vaccine containing structural proteins of hcv and evaluation of its expression in a eukaryotic system. methods: struct...

background: tuberculosis (tb) remains as a major cause of death around the world. construction of a new vaccine against tuberculosis is an effective way to control it. several vaccines against this disease have been developed. the aim of the present study was to cloning of tb10.4 gene in pcdna3.1+ plasmid and evaluation of its expression in eukaryotic cells. methods: firstly, tb10.4 fragment wa...