An Escherichia coli expression system was used to produce recombinant ricin A chain (RTA) and RTA modified either by the addition of a carboxyl-terminal endoplasmic reticulum retrieval sequence Lys-Asp-Glu-Leu (RTAKDEL) or a nonfunctional analogue Lys-Asp-Glu-Ala (RTAKDEA). These RTA molecules can enter mammalian cells by fluid phase endocytosis. RTAKDEL was significantly more cytotoxic than ei...

Bivalent histone modifications are defined as repressive and activating epigenetic marks that simultaneously decorate the same genomic region. The H3K27me3 mark silences gene expression while H3K4me3 mark prevents the region from becoming permanently silenced and prepares the domain for activation when needed. Specific regions of Kaposi's sarcoma-associated herpesvirus (KSHV) latent episomes ar...

road traffic accidents (rta) are one of the most important causes of death and disability all over the world. in developing countries the problem is more complicated. based on the estimation by unicef, rate of rta in iran is 20 times more than world’s average. in iran among all unintentional fatal injuries inflicted on children under five, traffic related fatalities are the leading cause of dea...

Background: Road Traffic Accident (RTA) is one among the top five causes of morbidity and mortality in South-East Asian countries including India. 1 Its socioeconomic repercussions are a matter of great concern. Efficient addressing of the issue requires quality information on different causative factors. ObjectivesWhat are different epidemiological determinants of RTA in Bhopal MP and examine ...

Viruses are obligate intracellular pathogens whose biological success depends upon replication and packaging of viral genomes, and transmission of progeny viruses to new hosts. The biological success of herpesviruses is enhanced by their ability to reproduce their genomes without producing progeny viruses or killing the host cells, a process called latency. Latency permits a herpesvirus to rema...

BACKGROUND Atrial fibrillation (AF) ablation is facilitated by anatomical visualization of the left atrium (LA) and the pulmonary veins (PVs). The purpose of this study was to compare accuracy, radiation exposure, and costs between three-dimensional atriography (3D-ATG) and cardiac computed tomography (CCT). METHODS Seventy patients with an indication for AF ablation were included. Contrast-e...

Rta, an Epstein-Barr virus (EBV)-encoded immediate-early protein, governs the reactivation of the viral lytic program by transactivating a cascade of lytic gene expression. Cellular transcription factors such as Sp1, ATF2, E2F, and Akt have been demonstrated to mediate Rta transactivation of lytic genes. We report herein that Rta associates with another potent transcription factor, tumor suscep...

BACKGROUND Fatal road traffic accidents (RTA) are a major cause of concern all over the world. The outcome of injuries sustained in an RTA depends on various factors including but not limited to: the location of the event, type of vehicle involved, nature of the roads, the time of accident, etc. AIMS This study aims to investigate and evaluate prospectively the socio-demographic profile and p...

Background: This research discusses the epidemiology of road traffic accidents which occurred in Ghana between 2001 and 2011. The purpose of this research is to provide a snapshot of road traffic accident cases which would inform the authorities on the need to ensure sanity on the roads. Methods: A descriptive and crosssectional study was adopted to explore the incidence of RTA in Ghana from 20...

Seventeen miRNAs encoded by Kaposi's sarcoma-associated herpesvirus (KSHV) have been identified and their functions have begun to be characterized. Among these miRNAs, we report here that miR-K12-7 directly targets the replication and transcription activator (RTA) encoded by open reading frame 50. We found that miR-K12-7 targeted the RTA 3' untranslated region (RTA3'UTR) in a seed sequence-depe...