Leaf and Cotyledon explants of Withania somnifera (L). Dunal were used to evaluate the effect of different growth regulators on the in vitro direct shoot and root initiation methods. Four different explants were used to establish callus shoot and root direct regeneration. In the first experiment leaf segments were cultured on MS basal supplemented with 2,4 – Dichlorophenoxyacetic acid (2,4 – D,...

We report the development of a cryopreservation protocol for the endemic Western Australian plant species Loxocarya cinerea (Restionaceae). Shoot tips from two genotypes, SXH404 and SXH804, were cryopreserved using the droplet-vitrification technique. Control explants, which were cryoprotected, but not cooled, showed regeneration for both genotypes (SXH404, 22.1 +/- 5.9%; SXH804, 67.7 +/- 9.6%)...

Citrus cultivars were explored for multiple shoot induction and root regeneration in different media. The multiple root and shoot induction was found directly proportionate to the increase in the levels of benzyleaminopurine (BA) and naphthalene acetic acid (NAA) in the modified Murashige and Skoog medium. The study might be promising towards in vitro propagation of sanitated Citrus plant mater...

The genetic control of gene expression during shoot development in Arabidopsis thaliana was analyzed by combining quantitative trait loci (QTL) and microarray analysis. Using oligonucleotide array data from 30 recombinant inbred lines derived from a cross of Columbia and Landsberg erecta ecotypes, the Arabidopsis genome was scanned for marker-by-gene linkages or so-called expression QTL (eQTL)....

Cryopreservation has been recognized as a practical and efficient tool for long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of sucrose concentration, hardening temperature and different cryopreservation methods on the survival rate of potato shoot tips after cryopreservation. Excised shoot tips of in vitro plantlets of potato cultivars, At...

A novel combination of plant growth regulators comprising indole-3-butyric acid (IBA), 6-benzylaminopurine (BA) and gibberellic acid (GA3) in Murashige and Skoog basal medium has been formulated for in vitro induction of both shoot and root in one culture using cotyledonary node explants of guar, (Cyamopsis tetragonoloba). Highest percentages of shoot (92%) and root (80%) induction were obtaine...

Sunflower (Helianthus annuus) is a crop of increasing importance as source seed oil and proteins; nonetheless, the number studies on sunflower tissue culture somewhat limited. The development competent in vitro direct organogenesis protocol involves important basic steps regeneration. In our study, chemically sterilized seeds were planted induction media, 52.54 % germination efficiency was foun...

Heliotropium kotschyi (Ramram), is a very important plant in relation to environment protection and for its medicinal value in Bahrain. It has been detected as one of the endangered plants in Bahrain. Ex situ conservation of this plant applying tissue culture method was used in the present study. In vitro plant regeneration has been established via shoot organogenesis using nodal meristems. The...

Pistacia vera cv Badami-e-riz and P.vera cv Ghazvini are the most important and popular rootstocks in Iran, which tolerate salinity and phytophthora fungi. This study was conducted to evaluate the effects of various concentrations of polyamines and IBA on root regeneration of transplanted bare- rooted ‘Badami-e-riz’ and ‘Ghazvini’ pistachio rootstocks. The mean comparison between IBA and polyam...