We present recent results on elastic and inelastic low/medium energy electron interaction with tetrahydrofuran, C4H8O, tetrahydrofurfuryl alcohol, C5H10O2 and 3hydroxytetrahydrofuran, C4H8O2 molecules in gaseous phase. A comparative study of both absolute differential cross sections for elastic electron scattering and electron energy loss spectra, in a wide incident electron energy range from 5...

A method of determining deoxyribose derivatives in biological material is described. It has high sensitivity, and is particularly useful in that it can be applied to a large range of tissues for which the other available assays are unsuitable. This is because the method is applicable to complex mixtures of nucleotides in which such substances as ribonucleotides are present in very large excess ...

The ATP-dependent DNase of Hemophilus injfuenzae Rd acts as a DNA-dependent ATPase which splits ATP into ADP and inorganic phosphate in the presence of magnesium ions and DNA. The apparent K, for ATP in the ATPase reaction is 0.18 mM. Approximately 40 ATP molecules are utilized for each DNA phosphodiester bond cleaved. All of the common ribose and deoxyribose nucleoside 5’-triphosphates can rep...

The structure of the novel nucleoside antibiotic A201A has been determined by a combination of chemical and spectroscopic methods. It is composed of 6-dimethylaminopurine, 3-amino-3-deoxyribose, p-hydroxy-alpha-methylcinnamic acid, a novel unsaturated hexofuranose and 3,4-di-O-methylrhamnose. Structures have also been assigned to several related minor factors simultaneously isolated from the fe...

Positioning of reactive functional groups within a DNA duplex can enable chemical reactions that otherwise would not occur to an appreciable extent. However, few studies have quantitatively defined the extent to which the enforced proximity of reaction partners in duplex DNA can favor chemical processes. Here, we measured substantial effective molarities (as high as 25M) afforded by duplex DNA ...

In the reaction catalysed by deoxyribose 5-phosphate aldolase (2-deoxy-D-ribose 5-phosphate acetaldehyde-lyase, EC 4.1.2.4) from Salmonella typhimurium, almost complete equilibration of the methyl-group protons of the product, acetaldehyde, occurs before its release from the enzyme surface. This phenomenon does not allow the stereo-chemical course of the reaction to be determined by using hydro...

DNA polymerase ß mutator mutants Pol ß is one of the smallest eukaryotic polymerases. It is a simple, well-characterised system to study the correlation between polymerase structure and fidelity of DNA synthesis. Polß plays a crucial role in base excision repair (BER) as it removes 5’-deoxyribose phosphate and fills in short lesions in DNA (short patch synthesis). Furthermore, polß is implicate...

Nearly complete 1H, 13C and 15N NMR assignments have been obtained for a doubly labeled 14-base pair DNA duplex in solution both in the free state and complexed with the uniformly 15N-labeled Antennapedia homeodomain. The DNA was either fully 13C, 15N-labeled or contained uniformly 13C, 15N-labeled nucleotides only at those positions which form the protein-DNA interface in the previously determ...

Multicellular tumor spheroids (MTS) from seven murine solid tumors have been compared in terms of their growth rate and the fraction of the cells that are in cycle. Growth rates ranged from a low of 19 micron/day through a maximum of 106 micron/day, demonstrating a far wider range of growth rates than is observed when the same tumors are grown as monolayers. Through the use of an [125I]iodourid...