Dual-energy X-ray absorptiometry (DXA) is recognized as the reference method to measure bone mineral density (BMD) with acceptable accuracy errors and good precision and reproducibility. The World Health Organization (WHO) has established DXA as the best densitometric technique for assessing BMD in postmenopausal women and based the definitions of osteopenia and osteoporosis on its results. DXA...

The activity of alkaline phosphatase in the duodenal and jejunal enterocytes was determined in 8-week-old clinically healthy piglets with normal (12 animals, average body weight 15.30 + 0.49 kg) and retarded growth (12 animals, average body weight 5.30 + 0.75 kg). A densitometric analysis was used. A marked decrease in an average density of the alkaline phosphatase (P < 0.001) was observed in t...

A simple, fast, accurate and precise high performance thin layer chromatographic method has been developed for the simultaneous estimation of domperidone and lansoprazole in capsule formulation. This method allows the determination of 100-500 ng/spot of domperidone and 100-500 ng/spot of lansoprazole. The mobile phase composition was toluene:isopropyl alcohol:chloroform:acetonitrile (4:3:6:2). ...

We studied the precision of densitometric quantification of the protein zones resolved by cellulose acetate electrophoresis. Replicate analyses of patients' samples by a single technologist showed mean CVs ranging from 2.9% for serum albumin to 9.5% for alpha 1-globulin. There were marked differences in measurements obtained by replicate analysis of the same samples by two experienced technolog...

Cineangiograms of 138 patients who underwent percutaneous transluminal coronary angioplasty (PTCA) were analyzed with a computer-based coronary angiography analysis system. The results before and after dilatation are presented. In a first study group (120 patients), the severity of the obstructive lesions derived from the automatically detected contours was evaluated in absolute terms and in pe...

By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybr...