نتایج جستجو برای: denaturing

تعداد نتایج: 4945  

2016

Denaturing Gradient Gel Electrophoresis is a influential and feasible device for sequence examination of the variety of complicted natural microbial populations. For PCR amplification of 16S rDNA products and specific primers were used and analyzed by denaturing gel electrophoresis in gradient. Probes specific for the cluster Genusand were designed to bind to sequences within the region amplifi...

Journal: :Gut 2002
E Miyazaki J Kato M Kobune K Okumura K Sasaki N Shintani P Arosio Y Niitsu

BACKGROUND AND AIMS Iron is stored in hepatocytes in the form of ferritin and haemosiderin. There is a marked increase in iron rich haemosiderin in iron overloaded livers, and ferric iron in amounts exceeding the ferritin and haemosiderin binding capacity may promote free radical generation, causing cellular damage. The aim of this study was to characterise hepatic haemosiderin using four antib...

2003
JESSE P. GREENSTEIN JOHN T. EDSALL

Myosin is a protein distinguished by its very high viscosity and intense double refraction of flow. It is also one of the few proteins which possess titratable sulfhydryl groups even in the native state; in the presence of certain denaturing agents the number of such groups is very greatly increased. Study of the action of a large number of denaturing agents on myosin has revealed certain pheno...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 2004
Kimberly A Zuhlke Jennifer J Madeoy Jennifer Beebe-Dimmer Kirsten A White Angela Griffin Ethan M Lange Stephen B Gruber Elaine A Ostrander Kathleen A Cooney

PURPOSE A genome-wide scan of 175 hereditary prostate cancer families from the University of Michigan Prostate Cancer Genetics Project provided evidence of prostate cancer linkage to 17q markers near the BRCA1 gene. To examine the possibility that germ-line BRCA1 mutations were associated with hereditary prostate cancer, individuals from 93 families with evidence of linkage to chromosome 17q we...

2011
Yiling Luo Nadukkudy V. Eldho Herman O. Sintim T. Kwaku Dayie

Obtaining homogeneous population of natively folded RNAs is a crippling problem encountered when preparing RNAs for structural or enzymatic studies. Most of the traditional methods that are employed to prepare large quantities of RNAs involve procedures that partially denature the RNA. Here, we present a simple strategy using 'click' chemistry to couple biotin to a 'caged' photocleavable (PC) g...

Journal: :BioTechniques 1996
C Gelfi L Cremonesi M Ferrari P G Righetti

A method for unambiguous determination of point mutations in genomic DNA, based on electrophoresis in thin capillaries, is reported here. The method is based on the principle of temperature gradient gel electrophoresis (TGGE), a variant of denaturing gradient gel electrophoresis (DGGE), and exploits the differential melting of mutant and wild-type PCR-amplified DNA fragments during electrophore...

Journal: :The Journal of General Physiology 1942
M. L. Anson

1. Cyanide inhibits the oxidation of the SH groups of cysteine and denatured egg albumin by the uric acid reagent. 2. At pH 4.8 cysteine is oxidized by the uric acid reagent and by ferricyanide in the presence but not in the absence of added copper sulfate. 3. In neutral solution, the uric acid reagent oxidizes the SH groups of denatured egg albumin in the presence of urea but not in the presen...

Journal: :Pediatrics 2006
M Elske van den Akker-van Marle Hinke M Dankert Paul H Verkerk Jeannette E Dankert-Roelse

OBJECTIVES The purpose of this work was to assess the costs of 4 neonatal screening strategies for cystic fibrosis in relation to health effects. In each strategy, the first test was the measurement of serum concentration of immunoreactive trypsin. The second step consisted of either a second immunoreactive trypsin test (strategy 1) or a multiple mutation analysis (strategy 2). In strategies 3 ...

Journal: :Food Science and Human Wellness 2023

Food allergens are mainly naturally-occurring proteins with immunoglobulin E (IgE)-binding epitopes. Understanding the structural and immunogenic characteristics of allergenic is essential in assessing whether how food processing techniques reduce allergenicity. We here discuss impacts technologies on modification physicochemical, structural, properties proteins. Detection for characterizing ch...

Journal: :Applied sciences 2021

The present study was undertaken in a constructed wetland (CW), setup tourism house, for domestic wastewater treatment. influence of season variations on the abundance fecal indicator organisms (total coliforms and Escherichia coli) substrate roots plants inhabiting inlet outlet zones CW evaluated along three consecutive years. structure diversity bacterial communities associated to CW’s also a...

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