Because hepatocyte growth factor (HGF) is a member of the endothelium-specific growth factors, we hypothesized that HGF may play a role in cardiovascular disease. Therefore we first examined the role of local HGF production in endothelial cell (EC) growth. Addition of anti-HGF antibody to EC resulted in a significant decrease in EC number. Moreover, coculture of vascular smooth muscle cells (VS...

We have developed a coculture system with autologous human endometrial epithelial cells (AEEC) that retained many features of human endometrial epithelium. Implantation failure (IF; >3 previous cycles failed with 3-4 good quality embryos transferred) is a distressing condition in which 2-day embryo transfer repetition is the routine option. The objective of this study was to investigate the bas...

behaviors of simultaneous removal of no and so2 using the coculture of anaerobic denitrifying bacteria and sulfate reducing bacteria were investigated in a bench-scale biotrickling-filter. approximate 20 days were required to use the landfill leachate as the microbial seed to develop the biofilm on the surface of packing material. when the combined no/so2 removal biotrickling-filter was operate...

We here report a new culturing method to produce micro vascular-mimetic tissue consisting of smooth muscle cells (SMCs) and endothelial cells (ECs) toward the practical use of tissue engineering. To achieve the stable coculture, the requirements for SMC culture in microchannels are clarified by basic experiments. Then, a new microchip that satisfies those requirements was developed by bonding g...

Electrospinning In article 2202302 by Matt J. Kipper, Salman R. Khetani, and co-workers, novel methods are developed to electrospin decellularized porcine liver extracellular matrix (ECM) collagen I into nanofibers without needing synthetic polymer blends toward mimicking the complex composition topography of native ECM. Primary human hepatocytes display higher long-term functions on in monocul...

We report the spatiotemporal control of a 3D coculture by combining photocurable and chemically degradable hydrogels for coculture of embryonic stem (ES) cells with HepG2 cells. ES cells were encapsulated in micropatterned photocurable poly(ethylene glycol) (PEG) hydrogels. The cell-laden PEG hydrogels were then encapsulated in calcium-alginate (Ca-Alg) hydrogel containing HepG2 cells. The ES c...