poplar trees are becoming extremely important as fast growing species due to the increasing need for wood and decreasing productivity of some lands in iran. the present study aimed to find the effect of canopy of poplar clones as one of the most important effective factors on soil properties compared to the distances inter-row trees. five poplar clones and species included: populus euramericana...

A bovine fibronectin (FN) cDNA clone (pFB1) was isolated by screening a cDNA library of calf testis fibroblasts with a synthetic oligonucleotide probe. The probe was a mixture of eight 14-base-long oligonucleotides designed from the amino acid sequence Glu-Cys-Phe-Met-Pro present in the Mr 3,000 COOH-terminal fragment of bovine plasma FN [Petersen, T.E., Thøgersen, H.C., Skorstengaard, K., Vibe...

A cosmid gene library of the genome of Lactococcus lactis subsp. lactis 712 has been constructed in the broad host range plasmid pLAFR1 in Escherichia coli LE392. Three lactococcal genes from the bank were identified by heterologous complementation of specific mutations in strains of E. coli. A cosmid clone encoding a putative lactose transport gene was identified by complementing an E. coli la...

Source and Description: Lambda clone 135 derived from a YAC from the CEPH library, mapping to an interval containing at least part of the Kallmann locus (1). DNA sequences flanking a (C-A)17 repeat of the lambda clone were used to design the polymerase chain reaction (PCR) primers. The polymorphism can be typed using PCR as previously described (2). Polymorphism: Five allelic fragments were res...

We have isolated two cDNA clones for potato {Solatium tuberosum L. cv. Kennebec) alcohol dehydrogenase (ADH, EC. 1.1.1.1) from a tuber cDNA library (1). Each clone encodes a polypeptide of 380 amino acids that differ at only one position (amino acid 5). The nucleotide sequences show 96% identity These two sequences are highly similar with the sequence of another potato ADH cDNA clone (1) and th...

We report the complete genomic sequence of the murine haemopoietic stem cell inhibitor gene (SCI) (1). The SCI protein is identical to the previously identified macrophage inflammatory protein la (MlP-la) (1,2). Using a MIP-la cDNA clone (2), we isolated a single clone from a murine (DBA/2J) adult liver genomic library (Clonetech Labs., USA) which contains the complete SCI/MIP-la gene. The amin...

A cDNA clone coding phenylalanine ammonia-lyase (PAL) was isolated from a cDNA library prepared from asparagus spears (Asparagus officinalis L. cv. Welcome) using the reverse transcription-polymerase chain reaction (RT-PCR). The partial cDNA clone encoded an mRNA of 527 bp and the derived amino acid sequence was found highly homologous to PAL from rice, maize and barley. Northern blot analysis ...

Xanthomonas oryzae pv. oryzae is an important plant pathogen which causes bacterial blight of rice. To facilitate genome studies of this bacterium, we have constructed a bacterial artificial chromosome (BAC) library of strain MAFF 311018. It consisted of 750 clones representing 16 genome equivalents, and had an insert size ranging from 20 to 220 kb with an average size of 107 kb. This library i...

A cDNA clone that encodes a Drosophila homologue of ribosomal protein L3 was isolated from a Drosophila ovary gridded cDNA library. The Drosophila ribosomal protein L3 gene (RpL3) is highly conserved with ribosomal protein L3 genes in other organisms. It is a single copy gene and maps to position 86D5-10 on polytene chromosomes. A Minute gene in this region, M(3) 86D, is a possible candidate to...

We have isolated several cDNA clones from a lambda gt11 expression library by screening with antibodies prepared against the microsomal enzyme steroid sulfatase, which is deficient in classical X-chromosome-linked ichthyosis patients. One of these clones (p422) has been assigned by mapping with a somatic cell hybrid panel and by in situ hybridization to Xp22.3. Clone p422 therefore has a coinci...