The aim of our research was to develop a chromatographic method for simultaneous determination of five substances used as disinfectant agents. Biphenyl-2-ol (BPh), chloramine T (ChT), 4-chloro-3-methylphenol (4C3MPh), triclosan (TCS), and triclocarban (TCC) were determined using high-performance liquid chromatography. Chromatographic analyses were carried out on a Develosil RP Aqueous AR-5 RP-3...

Divalent cationic strontium (Sr2~-l adsorption to and desorption from iron corrosion products were examined in two model drinking water distribution systems (DWDS). One system was maintained with chlorine-disinfected drinking water and the other with the same water with secondary chloramine disinfection. Flow conditions simulated primary transmission lines (constant flow) and residential mains ...

Reactive oxygen/nitrogen species are readily generated in vivo, playing roles in many physiological and pathological conditions, such as Alzheimer's disease and Parkinson's disease, by oxidatively modifying various proteins. Previous studies indicate that large conductance Ca(2+)-activated K(+) channels (BK(Ca) or Slo) are subject to redox regulation. However, conflicting results exist whether ...

The flavin-dependent halogenase RebH catalyzes the formation of 7-chlorotryptophan as the initial step in the biosynthesis of antitumor agent rebeccamycin. The reaction of FADH2, Cl-, and O2 in the active site generates the powerful oxidant HOCl, which was presumed to carry out the chlorination reaction. Herein, we demonstrate the formation of a long-lived chlorinating intermediate (t1/2 = 63 h...

Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. EGF preparations that had been iodinated with Chloramine T, lodogen, or lodo-beads were found mainly to consist of oxidized 125I-labeled EGF moieties. In contrast, the heterogeneous 125I-labeled EGF preparations obt...