نتایج جستجو برای: capture elisa

تعداد نتایج: 163920  

2003
Malcolm Alderton Brian Paddle

A sensitive and reproducible enzyme linked immunosorbent assay (ELISA) has been developed for the detection of ricin. The assay was developed using mouse polyclonal anti-ricin antibodies produced at AMRL and commercially available antibodies. The most efficient ELISA, using a goat anti-ricin antibody as the capture antibody, a rabbit anti-ricin as the second antibody and an alkaline phosphatase...

2013
S Murias Loza RM Alcobendas Rueda A Remesal Camba D Pascual Salcedo J Peralta R Merino Muñoz

Methods Cross-sectional retrospective study determining: 1) serum adalimumab (Ada) levels by a capture ELISA (positive > 5 ng/ml) and 2) levels of AAA by a two-site bridging ELISA [positive > 10 Arbitrary Units (AU)/ml]. The clinical activity was assessed by conventional tests and the physician visual analogue scale (ph-VAS) of 0 to 10, the same day that the blood sample was collected. Blood sa...

E Ansari.Dezfouli , G Mosahebi , M Koohi Habibi , S Mostafae ,

Abstract: Potato virus Y a type species of the genus potyvirus infects several crops in the family solanaceae. PVY isolated from field infected peppers was identified on the basis of host reaction, serological and molecular characterization. The result of ELISA, Immunoblot electrophoresis and Immuno Capture Reverse Transcription Polymerase Chain Reaction   (IC-RT-PCR) indicated that...

Journal: :iranian journal of veterinary research 2008
a. mohammadi k. asasi m. masoudian b. bozorgchami

avian leukosis viruses (alvs) cause different types of tumours in poultry and can affect the health and egg production of the birds. to investigate the presence of the virus in chicken layer flocks in shiraz, 222 egg albumen from local layer breeder (25 eggs), local layer grand parent (30 eggs), broiler breeder (60 eggs), commercial layer (46 eggs) and broiler grand parent (61 eggs) were tested...

2018
André Filipe Pastor Abraham Rocha Klécia de Melo Cassemiro Marli Tenório Paula Melo Maria Rosângela Grilis Maressa Rhuama Antonio Mauro Rezende Osvaldo Pompilio de Melo Ernesto Marques Rafael Dhalia

BACKGROUND Lymphatic filariasis (LF) is a parasitic disease caused mainly by the Wuchereria bancrofti worm and that affects up to 120 million people worldwide. LF is the second cause of chronic global deformity, responsible for 15 million people with lymphedema (elephantiasis) and 25 million men with scrotal hydrocele. Its diagnosis is still associated with numerous difficulties, such as the sa...

Journal: :Archives of disease in childhood 1996
G L Toms R Quinn J W Robinson

Sequential nasopharyngeal secretions were collected from 81 infants from one day to three months after admission to hospital with respiratory syncytial virus (RSV) infection. Samples from 21 infants were assayed for anti-RSV IgE in an antigen capture ELISA assay. No IgE antibodies were detected although an assay of IgA antibodies carried out in parallel by a similar technique detected IgA antib...

Journal: :FEMS microbiology immunology 1991
P M Richalet-Sécordel M H Van Regenmortel

A new capture test utilizing conjugated peptides has been developed for the detection of antibodies elicited against HIV-1. Human sera diluted 1:1000 were incubated in ELISA plates precoated with protein G. The captured IgG were allowed to react with three synthetic peptides corresponding to the gp41 sequence (591-611) YLKDQQLLGIWGCSGKLICTT, the gp120 sequence (314-329) IRIQRGPGRAFVTIGK and the...

Journal: :Journal of the American Society of Nephrology : JASN 2003
Kerstin W A Westman Daina Selga Per-Erik Isberg Anna Bladström Håkan Olsson

Wegener granulomatosis (WG) and microscopic polyangiitis (MP), diseases associated with antineutrophil cytoplasmic antibodies (ANCA), had an extremely poor prognosis before the introduction of cyclophosphamide and corticosteroids for their treatment. However, there is still reduced patient survival, and some studies have documented severe side effects of the immunosuppressants used. This 10-yr ...

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