Novel diazirinyl photoaffinity ligand, which contains (3-trifluoromethyl) phenyldiazirine and penta(epsilon-Boc-Lys) as a photoreactive code, allows the introduction of a positive cascade to alter the pI value of labeled components, facilitating the isolation of photolabeled biocomponents with isoelectric focusing techniques.

A mutant of Bacillus subtilis IMR-NK1, which is used for the production of domestic "natto" in Taiwan, produced high fibrinolytic enzyme activity by solid-state fermentation using wheat bran as medium. In addition, a strong fibrinolytic enzyme was purified from the cultivation media. The purified enzyme was almost homogeneous, as examined by SDS-PAGE and capillary electrophoresis. The enzyme ha...

A message passing interface (MPI) based parallel simulation algorithm is developed to simulate protein behavior in non-linear isoelectric focusing (IEF). The mathematical model of IEF is formulated based on mass conservation, charge conservation, ionic dissociation-association relations of amphoteric molecules and the electroneutrality condition. First, the concept of parallelism is described f...