Successful efficient whole cauliflower plant regeneration via somatic embryogenesis from root derived callus tissue was achieved. The research confirmed for the first time the capability of mass production of cauliflower somatic embryos through the indirect pathway. The best callus induction and proliferation was on semi solid Murashige and Skoog (MS) medium supplemented with 2, 4-D at 0.15 mg ...

Background Picrorhiza kurroa (PK) belongs to Scrophulariaceae family and is a representative endemic, medicinal herb, widely distributed throughout the higher altitudes of alpine Himalayas from west to east, between 3000 and 4500 m above mean sea level. Objective The objective of the present study is to assess the production of picroside I and picroside II from tissue cultures of PK. Materi...

Callus cultures established from Phaseolus vulgaris seedlings were used to investigate hormonal influence on lectin biosynthesis. The plant tissue cultures were initiated using defined levels of both a cytokinin (kinetin) and an auxin (2,4-dichlorophenoxyacetic acid) and were then transferred to media containing different amounts of these hormones. The lectin content of each callus culture was ...

Grapevine (Vitis vinifera L.) is one of the most economically important fruit crops in the world. Deficit in nitrogen, phosphorus and sulfur nutrition impairs essential metabolic pathways. The influence of mineral stress in the composition of the plant cell wall (CW) has received residual attention. Using grapevine callus as a model system, 6 weeks deficiency of those elements caused a signific...

Background: Sugarcane is a monocotyledonous crop that is cultivated in the tropical and subtropical regions of the world. One of the most important criteria, influencing the efficiency of the sugarcane transformation is known to be related to physical and biological factors during the transformation procedure. Objectives: The objective of this research was to study the response of callus induc...

sugar type and growth regulators are essential fundamental components of tissue culture media. in this study, different concentrations of some carbohydrate and growth regulators during anther culture and producing somatic embryo of datura stramonium l. was investigated in ms medium. the ms medium supplemented with 0.5 mgl-1 kinetin and 2 mgl-1 2,4-d, and also the medium supplemented with 0.25 m...

the natural lignan podophyllotoxin, a dimerized product of two phenylpropanoid moieties which occurs in a few plant species, is a pharmacologically important compound for its anticancer activities. optimization of the in vitro cultures might lead to a renewable source of lignans for medicinal uses. in order to maximize efficiency of podophyllotoxin accumulation via in vitro cultures, the effect...

Gyrinops versteegii is an endemic plant in eastern Indonesia that produced agarwood with high quality and economic value. This has been threatened by overexploitation which leads on decreasing the natural population. research aimed to induce vitro callus formation support sustainable utilization of G. versteegii, end, may productivity. Callus induction was investigated using cotyledon cultured ...

Embryogenic calli were induced from longitudinally bisected segments of protocorm-like bodies (PLBs) of Cymbidium Twilight Moon ‘Day Light’, a hybrid orchid, on modified Vacin and Went medium [Bot. Gaz. 110 (1949) 605] supplemented with 1-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) within 1 mont...

In 10-20% of the fractures, the fracture healing process is impaired which leads to non union and delayed union [1, 2]. Such factors have led to the quest for modalities which can enhance fracture healing. Fracture healing takes place through various stages as hematoma formation, inflammation, angiogenesis, callus formation, and bone remodeling. The healing cascade is coordinated by the interac...