Azf1 activates CLN3 transcription in Saccharomyces cerevisiae cells growing in glucose. Paradoxically, other studies have shown Azf1 to be almost undetectable in glucose-grown cells. Microarray experiments showed that Azf1 activates nonoverlapping gene sets in different carbon sources: in glucose, Azf1 activates carbon and energy metabolism genes, and in glycerol-lactate, Azf1 activates genes n...

BACKGROUND Solitary lung nodules in humans caused by the dog parasite Dirofilaria immitis are steadily increasing in number. The organisms can be easily missed in haematoxylin and eosin stained tissue when they are degenerated and pale staining. METHODS The value of Tinopal CBS-X (TCBS-X) and Calcofluor white (CFW), two rapid, inexpensive, simple non-specific fluorescent whitening stains, wer...

Polydimethylsiloxane (PDMS) chips have proven to be suitable environments for the growth of several filamentous organisms. However, depending on specimen, number investigations concerning their and cell differentiation is limited. In this work, we monitored developmental pattern brown alga Ectocarpus inside PDMS lab-on-chips. Two main methods inoculation lab-on-chip were tested, i.e., via direc...

Background: Invasive mould infections are associated with profound morbidity. Direct microscopic examination of specimens is a useful tool for laboratories to advise clinicians on the presumptive identity. Nonetheless, pitfalls abound. Here, we highlight how microscopy may not always be straightforward. Case description: A 69-year-old male T cell lymphoma was admitted chemotherapy. Shortly afte...

63 Chitin and β-1,3-glucan are known to play an important role in determination of fungal cell wall rigidity in Saccharomyces cerevisiae 1. Three chitin synthases participate in yeast chitin synthesis. ChS1p functions as a reparation enzyme after cell division 2 , ChS2p is responsible for the synthesis of primary septum 3, 4 and ChS3p synthesizes chitin in the lateral wall and in the ring at th...

In an interlaboratory study we compare different methods to determine the weight-average molecular weight (Mw) and distribution of six cereal beta-glucan isolates nutritional importance. Size-exclusion chromatography (SEC) with multi-angle light scattering (MALS), capillary viscometry, sedimentation velocity analytical ultracentrifugation one asymmetric flow field-flow fractionation (AF4)-MALS ...