methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...

mdma generally known as ecstasy, have deleterious effects on the serotonergic neurotransmitter system. recent findings suggest that the liver and brain are major target organs of mdma-related toxicities. although most research is being dynamically performed on brain, however, the molecular mechanisms by which mdma elicits adverse effects in both organs are poorly undrestood.the present study wa...

background 3-4methylenedioxymethamphetamine (mdma) is a synthetic and psychoactive drug, which is known popularly as ecstasy and has toxic effects on human organs. objectives considering the potential toxic interaction, this study was performed to quantify the expression of bax and bcl2 genes in mdma-induced hepatotoxicity on rat liver. subsequently, we evaluated pentoxifylline as a possible pr...

rapid detection and differentiation of infectious bronchitis virus (ibv) involved in the disease outbreak is very important for controlling disease and developing new vaccines. in the present study, three regions of the genome of ibv vaccine and field isolates including s1 gene, gene 3 and nucleocapsid (n) gene along with 3' untranslated region (3' utr) were amplified and subjected to restricti...

objective: today, aids is considered as a global problem and many efforts to generate an effective vaccine against this disease have been made, but remain inconclusive. dna vaccines are a member of the new generation of vaccines that can efficiently stimulate the immune system. however, recent findings indicate low immunogenicity for these vaccines and it is believed that these types of vaccine...

Most human gd T cells express Vg2Vd2 TCRs and play important roles in microbial and tumor immunity. Vg2Vd2 T cells are stimulated by self-and foreign prenyl pyrophosphate intermediates in isoprenoid synthesis. However, little is known about the molecular basis for this stimulation. We find that a mAb specific for butyrophilin 3 (BTN3)/CD277 Ig superfamily proteins mimics prenyl pyrophosphates. ...

objective(s) production of extended-spectrum beta-lactamases (esbls) by enteric bacteria continues to be a major problem in hospitals and community. esbls producing bacteria cause many serious infections including urinary tract infections, peritonitis, cholangitis and intra-abdominal abscess. the aim of this study was to determine the prevalence of esbls producing escherichia coli and klebsiell...

background: radiation therapy is among the most conventional cancer therapeutic modalities with effective local tumor control. however, due to the development of radio-resistance, tumor recurrence and metastasis often occur following radiation therapy. in recent years, combination of radiotherapy and gene therapy has been suggested to overcome this problem. the aim of the current study was to e...